Recombinant Human PLUNC BPIFA1 His-tag Protein, CF Summary
Product Specifications
Gln20-Val256, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
11221-BF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Measured by its ability to bind fluorescein conjugated E. coli bio-particles. The ED50 for this effect is 0.500-5.00 μg/mL.
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2 μg/lane of Recombinant Human PLUNC BPIFA1 His-tag Protein (Catalog # 11221-BF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 21-28 kDa.
Reconstitution Calculator
Background: PLUNC
BPIFA1 (BPI fold-containing family A member 1), also named SPLUNC1, is a member of the BPI-fold (BPIF) containing/Plunc (palate, lung, and nasal epithelium clone) superfamily of putative innate defense molecules which are predominantly expressed in regions of the oral cavity, nasopharynx, and upper respiratory tract (1, 2). BPIF proteins exist as two subgroups, BPIFA (formally SPLUNCs) and BPIFB (formally LPLUNCs) (1, 3). BPIFA proteins have structural homology to the N‑terminal domain of BPI whereas BPIFB proteins have structural homology to both domains of BPI (2). The mature human BPIFA1 shares 68% amino acid (aa) identity with mouse and rat BIPFA1. BPIF proteins appear to exhibit distinct tissue and cell specific expression patterns with various family members, being localized to several glandular structures within the upper respiratory tract, nasopharyngeal regions and oral cavity where they are secreted from these tissues and are found in high levels in saliva and nasal and respiratory lining fluids (2). BPI proteins play a role in diverse functions, including neutralization of endotoxin (LPS) in septic shock patients, inhibition of endothelial cell growth, dendritic cell maturation, and function as an anti-angiogenic, chemoattractant or opsonization agent (2). BPIFA1 and BPIFB1 expression was increased in late-stage chronic obstructive pulmonary disease (COPD) patients, and elevated levels correlate with disease severity (4). BPIFs are also upregulated in cystic fibrosis (CF) lung disease and may play a role in the pathogenesis of the disease (5).
- Bingle, C.D. and C. J. Craven (2002) Hum. Mol. Genet. 11:937.
- Alves, D.B. et al. (2017) Braz. Oral Res. 31:e6.
- Bingle, L. et al. (2012) Histochem. Cell Biol. 138:749.
- De Smet, E.G. et al. (2017) Int. J. Chron. Obstruct. Pulmon. Dis. 13:11.
- Saferali, A. et al. (2015) Am. J. Respir. Cell. Mol. Biol. 53:607.
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