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Recombinant Human Poly-SUMO3 Hydrolysis Resistant (3-8), CF

Catalog #: ULN-320 Datasheet / COA / SDS

Discontinued Product

ULN-320 has been discontinued.
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Recombinant Human Poly-SUMO3 Hydrolysis Resistant (3-8), CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
The function of SUMO chains is an area of intense research. K11-linked Hydrolysis-resistant Poly-SUMO3 Chains (3-8) are 500-fold more resistant to SENP disassembly than wild type chains and may be useful for investigating SUMO-binding proteins. Reaction conditions will need to be optimized for each specific application.
Source
E. coli-derived human SUMO3 protein

Each SUMO3 contains a Pro substitution at position 89.

Accession #
P55854
Predicted Molecular Mass

31 kDa (SUMO33), 42 kDa (SUMO34), 52 kDa (SUMO35), 63 kDa (SUMO36), 73 kDa (SUMO37), 84 kDa (SUMO38)

Product Datasheets

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ULN-320

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

ULN-320

Formulation

X mg/ml in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM DTT

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SUMO3

Sentrin protease (SENP)-resistant poly-SUMO3 chains can be used to investigate mechanisms of chain recognition by SUMO-targeted ubiquitin ligases (STUbl's) or other proteins that contain SUMO-interacting domains (SIM's). These K11-linked chains are formed enzymatically with recombinant SUMO3 containing a mutation of glutamine 89 to proline, which renders them approximately 500-fold more resistant to disassembly by SENP's than are wild-type chains. Mono- and di-SUMO3 have been removed from the chain mixture.

References
  1. Bekes, M., et al. (2011), J. Biol. Chem. 286: 10238
  2. Bekes, M., et.al. (2013) Cell Rep. 5: 826
  3. Bylebyl G.R., et al. (2003) J. Biol. Chem. 278: 44113
  4. Dohmen R. J. (2004) Biochem. Biophys. Acta. 1695:113
  5. Johnson E. (2004) Ann. Rev. Biochem. 73: 355
  6. Tatham M.H., et al. (2001) J. Biol. Chem. 276: 35368
Long Name
Small Ubiquitin-like Modifier 3
Entrez Gene IDs
6612 (Human)
Alternate Names
SMT3 (suppressor of mif two 3, yeast) homolog 1; SMT3 homolog 1; SMT3 suppressor of mif two 3 homolog 1; SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae); SMT3 suppressor of mif two 3 homolog 3 (yeast); SMT3A; SMT3B; SMT3H1; SMT3H1small ubiquitin-related modifier 3; SUMO-2; SUMO3; SUMO-3; ubiquitin-like protein SMT3A; Ubiquitin-like protein SMT3B

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