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Recombinant Human Poly-Ub WT Chains (3-7) (K63-linked), CF

Catalog #: UC-320
5 Citations Datasheet / COA / SDS

Discontinued Product

UC-320 has been discontinued.
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Recombinant Human Poly-Ub WT Chains (3-7) (K63-linked), CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Ubiquitin chains vary in length, linkage, and function. K63-linked Poly-Ubiquitin Chains (Ub3-7) are ideal for investigating Ubiquitin-binding proteins and as substrates for Ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40 °C for 20 minutes prior to gel electrophoresis.
Source
E. coli-derived human Poly-Ubiquitin protein
Accession #
P0CG47
Predicted Molecular Mass
26 kDa (Ub3), 34 kDa (Ub4), 43 kDa (Ub5), 52 kDa (Ub6), and 60 kDa (Ub7)

Product Datasheets

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UC-320

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UC-320

Formulation Lyophilized from a solution in deionized water.
Reconstitution Reconstitute at 2 mg/mL in an aqueous solution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Poly-Ubiquitin

Poly-Ubiquitin chains are composed of Ubiquitin monomers that are covalently linked through  isopeptide bonds, which typically form between a lysine residue of one Ubiquitin molecule and the C-terminal glycine residue of another Ubiquitin molecule (1). Each human Ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse Ubiquitin, respectively (2). Seven of the 76 aa in Ubiquitin are lysine residues that can participate in poly-Ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes (3-8).

Linkage specific poly-Ubiquitin chains are used to investigate mechanisms of chain recognition, binding and hydrolysis by the proteasome, deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). Lys63-linked poly-Ubiquitin has been implicated in several non-degradative processes such as receptor endocytosis and sorting, translation, DNA damage repair, the stress response and signaling in theNF kappa B pathway. This product is formed with wild-type ubiquitin and linkage-specific enzymes.This mixture of poly-Ubiquitin chains contains tri-Ubiquitin and higher MW species, mono- and di-Ubiquitin have been removed.

References
  1. Scheffner, M. et al. (1995) Nature 373:81.
  2. Sharp, P.M. & W.-H. Li (1987) Trends Ecol. Evol. 2:328.
  3. Behrends, C. & J.W. Harper (2011) Nat. Struct. Mol. Biol. 18:520.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Henry, A.G. et al. (2012) Dev. Cell 23:519.
  6. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  7. Wei, W. et al. (2004) Nature 428:194.
  8. Zhang, J. et al. (2012) J. Biol. Chem. 287:28646.
Alternate Names
CEP80; HEL112; PolyUbiquitin; Poly-Ubiquitin; ribosomal protein S27a; RPS27A; S27A; UBA80; UBC; UBCEP1; UBCEP80

Citations for Recombinant Human Poly-Ub WT Chains (3-7) (K63-linked), CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Characterizing the PRRSV nsp2 Deubiquitinase Reveals Dispensability of Cis-Activity for Replication and a Link of nsp2 to Inflammation Induction
    Authors: S Zhou, X Ge, C Kong, T Liu, A Liu, P Gao, J Song, L Zhou, X Guo, J Han, H Yang
    Viruses, 2019-09-26;11(10):.
    Species: Virus - PRRSV
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome Stability
    Authors: P Haahr, N Borgermann, X Guo, D Typas, D Achuthanku, S Hoffmann, R Shearer, TK Sixma, N Mailand
    Mol. Cell, 2018-03-22;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  3. OTUD4 Is a Phospho-Activated K63 Deubiquitinase that Regulates MyD88-Dependent Signaling
    Authors: Y Zhao, MC Mudge, JM Soll, RB Rodrigues, AK Byrum, EA Schwarzkop, TR Bradstreet, SP Gygi, BT Edelson, N Mosammapar
    Mol. Cell, 2018-02-01;69(3):505-516.e5.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. USP7 is a SUMO deubiquitinase essential for DNA replication
    Authors: E Lecona, S Rodriguez-, J Specks, AJ Lopez-Cont, I Ruppen, M Murga, J Muñoz, J Mendez, O Fernandez-
    Nat Struct Mol Biol, 2016-03-07;23(4):270-7.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  5. A20 inhibits LUBAC-mediated NF-kappaB activation by binding linear polyubiquitin chains via its zinc finger 7.
    EMBO J., 2012-08-28;31(19):3845-55.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay

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