Recombinant Human Protocadherin-17 Protein, CF

Catalog #: 7669-CA Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7669-CA-050

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Recombinant Human Protocadherin-17 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED₅₀ for this effect is 0.06-0.24 ug/mL.

Source

Human embryonic kidney cell, HEK293-derived human Protocadherin-17 protein
Leu18-Ser705, with a C-terminal 6-His tag

Accession #

O14917

N-terminal Sequence
Analysis

Leu18

Predicted Molecular Mass

75.1 kDa

SDS-PAGE

80-105 kDa, reducing conditions

Product Datasheets

7669-CA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7669-CA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Protocadherin-17

Human Protocadherin 17 (PCDH17; also called Protocadherin‑68) is an approximately 1159 amino acid (aa) glycoprotein belonging to the δ2 subgroup of protocadherins, type I transmembrane proteins that exhibit calcium‑dependent but relatively weak homophilic adhesion (1‑3). Like other δ2 protocadherins, PCDH17 cDNA encodes a signal sequence (aa 1‑17), six cadherin domains of about 100 aa each (aa 18‑695), a transmembrane sequence and a cytoplasmic domain (1). It contains an extracellular RGD cell attachment motif that may mediate integrin adhesion (3). Typical of protocadherins, an alternately spliced 889 aa form of human PCDH17 diverges at aa 876 within the cytoplasmic domain and lacks two conserved cytoplasmic motifs (1, 2). Within the extracellular domain, human PCDH17 shares 98% aa sequence identity with mouse and bovine, 99% with rat and equine, and 97% with porcine PCDH17. PCDH17 is constitutively expressed in the central nervous system (3). It is one of several PCDH molecules that shows region specificity in the basal ganglia, forming gradients (3). Early in postnatal life, it is preferentially expressed in motor and auditory cortices and is correlated with thalamo‑cortical and intrahippocampal circuits (3‑5). Its expression is increased in the Broadman area of the brain in subjects with schizophrenia (3). PCDH17 is frequently silenced in squamous cell carcinomas such as those in the esophagus and larynx, especially if they are poorly differentiated (3, 6, 7). Ectopic PCDH10 expression reduces tumor cell proliferation and migration (6).

References