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Recombinant Human PSG6 Protein, CF

Catalog #: 9318-P6 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9318-P6-050
Recombinant Human PSG6 Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human PSG6 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.9-4.5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human PSG6 protein
Gln35-His424, with a C-terminal 6-His tag
Accession #
Q00889-2
N-terminal Sequence
Analysis
No results obtained. Gln35 inferred from enzymatic pyroglutamate treatment revealing Val36
Predicted Molecular Mass
45 kDa
SDS-PAGE
62-73 kDa, reducing conditions

Product Datasheets

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9318-P6

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9318-P6

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human PSG6 Protein Binding Activity View Larger

Recombinant Human PSG6 (Catalog # 9318-P6) induces adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.9-4.5 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PSG6

Pregnancy-specific glycoprotein 6 (PSG6), also known as PSG10 and PSGGB is a secreted glycoprotein of the PSG family within the CEA (carcinoembryonic antigen) superfamily (1). Mature human PSG6 is a 401 amino acid (aa) protein that consists of four tandem immunoglobulin-like domains followed by a 30 aa
C-terminal tail (2, 3). The N-terminal Ig-like domain contains an Arg-Gly-Asp (RGD) sequence which is a consensus integrin-binding motif found in many adhesion proteins (4, 5). Alternative splicing generates an additional isoform with a substitution in the C-terminal tail. PSG6 is predominantly expressed in placenta and more weakly in granulocytes (3). It induces monocyte production of the cytokines IL-6, IL-10, and TGF-beta 1 (6).

References
  1. Kuespert, K. et al. (2006) Curr. Opin. Cell Biol. 18:565.
  2. Zimmermann, W. et al. (1989) Biochem. Biophys. Res. Commun. 163:1197.
  3. Barnett, T.R. et al. (1990) Biochemistry 29:10213.
  4. Leslie, K.K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5822.
  5. Ruoslahti, E. (1996) Annu. Rev. Cell Dev. Biol. 12:697.
  6. Snyder, S.K. et al. (2001) Am. J. Reprod. Immunol. 45:205.
Long Name
Pregnancy-specific beta-1-glycoprotein 6
Entrez Gene IDs
5675 (Human)
Alternate Names
CGM3; pregnancy specific beta-1-glycoprotein 6; Pregnancy-specific beta-1-glycoprotein 10; Pregnancy-specific beta-1-glycoprotein 12; pregnancy-specific beta-1-glycoprotein 6; Pregnancy-specific glycoprotein 10; Pregnancy-specific glycoprotein 12; Pregnancy-specific glycoprotein 6; PS-beta-G-10; PS-beta-G-12; PS-beta-G-6; PSBG-10; PSBG-12; PSBG-6; PSG10; PSG12; PSG6; PSG-6; PSGGB

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