Home / RANK/TNFRSF11A / Recombinant Human RANK/TNFRSF11A Fc Chimera Protein, CF

Recombinant Human RANK/TNFRSF11A Fc Chimera Protein, CF

Catalog #: 683-RK
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
683-RK-100
R&D Systems Recombinant Proteins and Enzymes
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Citations (7)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human RANK/TNFRSF11A Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit TRANCE-induced osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 0.005‑0.035 µg/mL in the presence of 2 ng/mL of rmTRANCE (Catalog # 462-TEC).
Source
Mouse myeloma cell line, NS0-derived human RANK/TNFRSF11A protein
Met Human RANK
(Gln29-Gly213)
Accession # Q9Y6Q6.1		 IEGRDMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
Q9Y6Q6.1
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
48 kDa (monomer)
SDS-PAGE
60 kDa, reducing conditions

Product Datasheets

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683-RK

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

683-RK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RANK/TNFRSF11A

RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR]) and TNFRSF11A is a member of the tumor necrosis factor receptor family. The full length human RANK cDNA encodes a type I transmembrane protein of 616 amino acids with a predicted 184 amino acid extracellular domain and a 383 amino acid cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains. RANK is widely expressed with highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.

References
  1. Anderson, D.M. et al. (1997) Nature 390:175.
  2. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
Long Name
Receptor Activator of NF-kappa-B
Entrez Gene IDs
8792 (Human); 21934 (Mouse)
Alternate Names
CD265 antigen; CD265; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFR; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANK; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TNFRSF11A; TRANCE R; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator

Citations for Recombinant Human RANK/TNFRSF11A Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Soluble RANKL contributes to osteoclast formation in adult mice but not ovariectomy-induced bone loss
    Authors: J Xiong, K Cawley, M Piemontese, Y Fujiwara, H Zhao, JJ Goellner, CA O'Brien
    Nat Commun, 2018-07-25;9(1):2909.
    Species: Mouse
    Sample Types: Serum
    Applications: Bioassay
  2. A Modified Glycosaminoglycan, GM-0111, Inhibits Molecular Signaling Involved in Periodontitis
    Authors: Justin R Savage
    PLoS ONE, 2016-06-16;11(6):e0157310.
    Applications: Bioassay
  3. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Soluble decoy receptor 3 modulates the survival and formation of osteoclasts from multiple myeloma bone disease patients.
    Authors: Colucci S, Brunetti G, Mori G, Oranger A, Centonze M, Mori C, Cantatore FP, Tamma R, Rizzi R, Liso V, Zallone A, Grano M
    Leukemia, 2009-07-09;23(11):2139-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Lymphocytes and synovial fluid fibroblasts support osteoclastogenesis through RANKL, TNFalpha, and IL-7 in an in vitro model derived from human psoriatic arthritis.
    Authors: Colucci S, Brunetti G, Cantatore FP, Oranger A, Mori G, Quarta L, Cirulli N, Mancini L, Corrado A, Grassi FR, Grano M
    J. Pathol., 2007-05-01;212(1):47-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis.
    Authors: Edwards JR, Sun SG, Locklin R, Shipman CM, Adamopoulos IE, Athanasou NA, Sabokbar A
    Arthritis Rheum., 2006-05-01;54(5):1451-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Development of a novel surrogate virus for human T-cell leukemia virus type 1: inhibition of infection by osteoprotegerin.
    Authors: Okuma K, Dalton KP, Buonocore L, Ramsburg E, Rose JK
    J. Virol., 2003-08-01;77(15):8562-9.
    Species: Hamster, Human
    Sample Types: Whole Cells
    Applications: Bioassay

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