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Recombinant Human RECK His-tag Protein, CF

Catalog #: 10236-RE Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10236-RE-050
Recombinant Human RECK His-tag Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human RECK His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA.

When Recombinant Human RECK His-tag (Catalog # 10236-RE) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human TEM5/GPR124 His-tag binds with an ED50 of 0.7-4.9 µg/mL.

Source
Chinese Hamster Ovary cell line, CHO-derived human RECK protein
Gly27-Pro941, with a C-terminal 6-His tag
Accession #
O95980
N-terminal Sequence
Analysis
Gly27
Predicted Molecular Mass
102 kDa
SDS-PAGE
103 - 122 kDa, under reducing conditions

Product Datasheets

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10236-RE

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10236-RE

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human RECK His-tag Protein Binding Activity View Larger

When Recombinant Human RECK His-tag (Catalog # 10236-RE) is immobilized at 2 μg/mL (100 μL/well), Biotinylated Recombinant Human TEM5/GPR124 His-tag binds with an ED50 of 0.7-4.9 μg/mL.

SDS-PAGE Recombinant Human RECK His-tag Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human RECK His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at ~110 kDa under reducing conditions.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RECK

Reversion-inducing cysteine-rich protein with Kazal motifs (RECK), also known as Suppressor of tumorigenicity 15 protein (ST15), is a 971 amino acid highly conserved protein that serves as an important mediator of tissue remodeling (1). The multi-domain protein includes hydrophobic regions at the N- and C-termini that correspond to a signal domain and GPI anchoring site, respectively (2). Internally, there are three serine protease inhibitor-like (SPI) domains and two regions with EGF-like repeats (2). The N-terminal region also contains a cysteine-rich domain essential for MMP (3) and Wnt7 binding (4, 5). RECK is normally expressed in all human and mammalian cells (2, 6) while undetectable or downregulated in malignant and cancer cells (1, 2). There is a strong correlation with expression of RECK in several cancers including colorectal, breast, and pancreatic making it a prognostic marker of interest (1, 3, 7-9). Polymorphisms in RECK leads to increased cancer susceptibility (10). RECK's ability to bind several proteins confers complex functionality. RECK is known to bind and inhibit MMPs (1, 2, 11), interact with ADAMTS10 (12), modulate Notch signaling (13), promote p53 signaling (14) and act as a selective Wnt7 receptor through binding of Gpr124 (4). RECK acts as a tumor suppression gene by inhibiting angiogenesis, invasion, and metastasis through its role in the regulation and signaling within the extracellular matrix (1, 3, 6). Through its interaction with Wnt7, RECK promotes angiogenesis and regulates the blood-brain barrier in CNS by mediating canonical Wnt/beta-catenin signaling (4).

References
  1. Alexius-Lindgren, M. et al. (2014) Anticancer Res. 34:3867.
  2. Takahashi, C. et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:13221.
  3. Clark, J.C.M. et al. (2007) Cancer Mestast. Rev. 26:675.
  4. Vallon, M. et al. (2018) Cell Reports. 25:339.
  5. Cho, C. et al. (2019) Elife. 8:e47300.
  6. Oh, J. et al. (2001) Cell. 107:789.
  7. Takeuchi, T. et al. (2004) Clin. Cancer Res. 10:5572.
  8. Zhang, G. et al. (2012) Cancer Sci. 103:1084.
  9. Masui, T. et al. (2003) Clin. Cancer Res. 9:1779.
  10. Chung, T.T. et al. (2012) PLoS ONE. 7:e33517.
  11. Miki, T. et al. (2007) J. Biol. Chem. 282:12341.
  12. Matsuzaki, T. et al. (2018) Biol. Open 7:bio033985.
  13. Muraguchi, T. et al. (2007) Nat. Neurosci. 10:838.
  14. Lui, Y. et al. (2018) J. Cell. Biochem. 119:3058.
Long Name
Reversion-inducing-cysteine-rich Protein with Kazal Motifs
Entrez Gene IDs
8434 (Human); 53614 (Mouse)
Alternate Names
hRECK; membrane-anchored glycoprotein (metastasis and invasion); RECK; reversion-inducing-cysteine-rich protein with kazal motifs; ST15; ST15reversion-inducing cysteine-rich protein with Kazal motifs; suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein withkazal motifs); suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein withkazal motifs); Suppressor of tumorigenicity 15 protein

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