Recombinant Human RGM-C/Hemojuvelin Protein, CF Summary
- R&D Systems NS0-derived Recombinant Human RGM-C/Hemojuvelin Protein (3720-RG)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
10‑500 ng/mL.
Gln36-Asp400 (mature) & Pro173-Asp400 (C-terminus chain), both with a C-terminal 6-His tag & Gln36-Asp172 (N-terminus chain)
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3720-RG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: RGM-C/Hemojuvelin
RGM-C, also known as hemojuvelin, is a member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane glycoproteins (1, 2). RGM-C is expressed in striated muscle and periportal hepatocytes (3 - 5). The protein undergoes partial cleavage intracellularly, resulting in a disulfide-linked dimer of the 14 kDa N-terminal and 33 kDa C-terminal portions (4, 6, 7). The N-terminal fragment contains an RGD motif, while the C-terminal fragment carries the GPI attachment site (4, 7). Two alternatively spliced isoforms lack either approximately half or the entire N-terminal fragment. Full length RGM-C can also be released from the cell and circulates in the blood (6, 8). RGM-C is disrupted in type 2A juvenile hemochromatosis, a hereditary iron homeostasis disorder characterized by excessive iron accumulation (5). In mouse, loss of RGM-C function results in decreased expression of the iron regulatory hormone hepicidin and increased iron deposition in liver, pancreas, and heart (5, 9). Membrane associated RGM-C upregulates hepicidin while soluble RGM-C downregulates hepicidin expression (8). This appears to be an iron-responsive regulatory system, as high blood iron levels reduce the amount of soluble RGM-C produced (8). RGM-C, similar to RGM-A, associates with neogenin (7). Disease-related point mutations can prevent internal RGM-C cleavage or its ability to interact with neogenin (6, 7). Experimental inflammatory conditions result in decreased RGM-C expression and increased hepicidin expression, although the two effects occur independently (5, 10). RGM-C also functions as a BMP coreceptor and enhances BMP-2 and BMP-4 signaling (11). In this context, RGM-C enhances the BMP-2 upregulation of hepatic hepicidin (11). Mature human RGM-C shares 89% amino acid (aa) sequence identity with mouse and rat RGM-C. It shares 49% and 44% aa sequence identity with human RGM-A and RGM-B, respectively.
- Papanikolaou, G. et al. (2004) Nat. Genet. 36:77.
- Schmidtmer, J. and D. Engelkamp (2004) Gene Exp. Patterns 4:105.
- Oldekamp, J. et al. (2004) Gene Exp. Patterns 4:283.
- Niederkofler, V. et al. (2004) J. Neurosci. 24:808.
- Niederkofler, V. et al. (2005) J. Clin. Invest. 115:2180.
- Kuninger, D. et al. (2006) J. Cell Sci. 119:3273.
- Zhang, A.S. et al. (2005) J. Biol. Chem. 280:33885.
- Lin, L. et al. (2005) Blood 106:2884.
- Huang, F.W. et al. (2005) J. Clin. Invest. 115:2187.
- Krijt, J. et al. (2004) Blood 104:4308.
- Babitt, J.L. et al. (2006) Nat. Genet. 38:531.
Citations for Recombinant Human RGM-C/Hemojuvelin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
Repulsive guidance molecule (RGM) family proteins exhibit differential binding kinetics for bone morphogenetic proteins (BMPs).
Authors: Wu, Qifang, Sun, Chia Chi, Lin, Herbert, Babitt, Jodie L
PLoS ONE, 2012-09-27;7(9):e46307.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance -
Identification of retinoic acid in a high content screen for agents that overcome the anti-myogenic effect of TGF-beta-1.
Authors: Krueger C, Hoffmann FM
PLoS ONE, 2010-11-30;5(11):e15511.
Species: Human
Sample Types: Buffer, Serum
Applications: ELISA Developmet, Western Blot -
Immunoassay for human serum hemojuvelin.
Authors: Brasse-Lagnel C, Poli M, Lesueur C, Grandchamp B, Lavoinne A, Beaumont C, Bekri S
Haematologica, 2010-08-16;95(12):2031-7.
Species: Human
Sample Types: N/A, Serum
Applications: ELISA Developmet, Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human RGM-C/Hemojuvelin Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human RGM-C/Hemojuvelin Protein, CF and earn rewards!
Have you used Recombinant Human RGM-C/Hemojuvelin Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
