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Recombinant Human RNF4 Protein, CF

Catalog #: E3-210
2 Citations Datasheet / COA / SDS

Discontinued Product

E3-210 has been discontinued.
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Recombinant Human RNF4 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human RNF4 is a Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human RNF4 concentration of 100-500 nM.
Source
E. coli-derived human RNF4 protein
Accession #
P78317
Predicted Molecular Mass
22 kDa

Product Datasheets

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E3-210

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-210

Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 5 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RNF4

RNF4 (small nuclear ring finger protein, SNURF) is a RING-finger ubiquitin E3 ligase that ubiquitinates and mediates the proteasomal destruction of targets such as PML, PEA3, CENP1, and PARP1. In addition to the RING domain, RNF4 contains four SUMO-interacting motifs (SIMs) that function to recruit this ligase to poly-sumoylated substrates. RNF4 will autoubiquitinate in vitro, and will also ubiquitinate poly-SUMO chains.

References
  1. Geoffroy, M-C et al. (2010) Mol. Bio. Cell 21: 4227
  2. Tathum, M.H. et al. (2008) Nat. Cell Bio. 10: 538
Long Name
Ring Finger Protein 4
Entrez Gene IDs
6047 (Human)
Alternate Names
E3 ubiquitin ligase RNF4; EC 6.3.2.-; Protein SNURF; RES4-26; ring finger protein 4SNURFSLX5; RNF4; SLX5; Small nuclear ring finger protein; SNURF

Citations for Recombinant Human RNF4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation
    Authors: Y Sun, J Chen, SN Huang, YP Su, W Wang, K Agama, S Saha, LM Jenkins, JM Pascal, Y Pommier
    Nature Communications, 2021-08-18;12(1):5010.
    Species: Human
    Sample Types:
    Applications: Bioassay
  2. Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
    Authors: CW Davies, SE Vidal, L Phu, J Sudhamsu, TB Hinkle, S Chan Rosen, FR Schumacher, YJ Zeng, C Schwerdtfe, AS Peterson, JR Lill, CM Rose, AS Shaw, IE Wertz, DS Kirkpatric, JT Koerber
    Nature Communications, 2021-07-29;12(1):4608.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay

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