Recombinant Human Ryk Fc Chimera Protein, CF Summary
Product Specifications
| Human Ryk (Ala46-Thr224) Accession # NP_001005861 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4907-RK
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Ryk
Ryk (Related to tyrosine kinase) is an 70-90 kDa type I transmembrane glycoprotein that is an atypical member of the receptor tyrosine kinase superfamily (1, 2). Human Ryk cDNA encodes 604 amino acids (aa) that include a 25 aa signal sequence, a 199 aa extracellular domain (ECD, aa 26‑224) that contains a WIF (Wnt Inhibitory Factor) domain (aa 63‑191), a transmembrane sequence, and a cytoplasmic region with a nonfunctional Ser/Thr protein kinase domain (aa 327‑600) (1‑3). The cytoplasmic region participates in signaling by undergoing gamma ‑secretase cleavage and translocating to the nucleus in response to Wnts (4). An isoform with a 31 aa substitution between aa 18‑46 is reported. Within the ECD, human Ryk shares approximately 94%, 92%, 86% and 81% aa sequence identity with mouse, rat, equine and canine Ryk, respectively. Ryk protein expression is reported in developing neurons of the corticospinal tract, ventral zone, and retina, as well as adult tissue epithelia, stroma and blood vessels (4‑9). Ryk binds Wnts and EphB2/B3 receptors via its WIF domain (8‑10). It may form a Wnt receptor complex with Frizzled receptors, serving as a link between Wnt and Dishevelled and converting Wnt/Frizzled attraction signals to repulsion signals (1, 6‑8, 10). In particular, Ryk is required for Wnt5a‑induced axon guidance after cortical axons cross the corpus callosum, and for Wnt3-mediated guidance of developing retinal ganglion cells (7, 8). Mice deleted for the Ryk gene show defects in axon guidance that produce craniofacial defects and shortened limbs (10, 11). Ryk-/- mice share a cleft palate phenotype with EphB2/B3-deleted mice (10). Ryk is often overexpressed in ovarian cancer (5).
- Stacker, S.A. et al. (1993) Oncogene 8:1347.
- Clark, C.E.J. et al. (2012) Neurosignals 20:202.
- Katso, R.M. et al. (1999) Mol. Cell. Biol. 19:6427.
- Lyu, J. et al. (2008) Dev. Cell 15:773.
- Katso, R.M. et al. (1999) Cancer Res. 59:2265.
- Liu, Y. et al. (2005) Nat. Neurosci. 8:1151.
- Schmitt, A.M. et al. (2006) Nature 439:31.
- Keeble, T.R. et al. (2006) J. Neurosci. 26:5840.
- Kamitori, K. et al. (2005) Biochem. Biophys. Res. Commun. 330:446.
- Lu, W. et al. (2004) Cell 119:97.
- Halford, M.M. et al. (2000) Nat. Genet. 25:414.
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