Recombinant Human S100A11 Protein, CF Summary
Product Specifications
Ala2-Thr105
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9015-S11
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and DTT with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: S100A11
S100A11, also known as S100C and Calgizzarin, is a 10 kDa member of the S100 family of calcium binding proteins (1-3). Human S100A11 contains two EF hand motifs and shares 82% amino acid sequence identity with mouse and rat S100A11. It forms covalent homodimers upon transglutamination and also disulfide-linked tetramers (4, 5). S100A11 is secreted by keratinocytes and can be crosslinked into the cornified envelope of the skin (6). Dimerization enhances its ability to signal through RAGE on keratinocytes, induce the production of EGF family proteins, and induce cell proliferation (7). Dimerization also enables S100A11 to bind RAGE on chondrocytes, leading to chondrocyte hypertrophy and catabolism of the cartilage matrix (4). S100A11 is additionally found in the cytosol where it becomes phosphorylated and translocates to the nucleus in response to DNA damage, RELM alpha exposure, or elevated extracellular calcium concentrations (8-10). Calcium also promotes S100A11 association with S100B as well as Annexins A1, A2, and A6 (5, 11-13). S100A11-Annexin A2 complexes are recruited to sites of plasma membrane damage where they facilitate membrane repair in migrating cancer cells (13). S100A11 is upregulated in various cancers and supports tumor cell proliferation, invasion, and migration (7, 14). In addition, S100A11 is produced in the ovary, and it acts on cumulus cells to inhibit oocyte fertilization (15).
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- Cecil, D.L. and R. Terkeltaub (2008) J. Immunol. 180:8378.
- Chang, N. et al. (2007) Am. J. Physiol. Cell Physiol. 292:C1417.
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- Sakaguchi, M. et al. (2007) Mol. Biol. Cell 19:78.
- Gorsler, T. et al. (2010) BMC Cell Biol. 11:100.
- Fan, C. et al. (2011) Mol. Cell Proteomics 10:M110.000901.
- Sakaguchi, M. et al. (2003) J. Cell Biol. 163:825.
- Deloulme, J.C. et al. (2000) J. Biol. Chem. 275:35302.
- Mailliard, W.S. et al. (1996) J. Biol. Chem. 271:719.
- Jaiswal, J.K. et al. (2014) Nat. Commun. 5:3795.
- Liu, Y. et al. (2015) Exp. Ther. Med. 9:1460.
- Hanaue, M. et al. (2011) Mol. Reprod. Dev. 78:91.
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