Home / Semaphorin 5A / Recombinant Human Semaphorin 5A Fc Chimera Protein, CF

Recombinant Human Semaphorin 5A Fc Chimera Protein, CF

Catalog #: 5896-S5
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
5896-S5-025
Recombinant Human Semaphorin 5A Fc Chimera Protein SEC-MALS.
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Semaphorin 5A Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit proliferation of the A549 human lung carcinoma cells. The ED50 for this effect is 100-400 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Semaphorin 5A protein
Human Semaphorin 5A
(Glu23-Thr765)
Accession # AAC09473		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
AAC09473
N-terminal Sequence
Analysis
Glu23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
110 kDa (monomer)
SDS-PAGE
133 kDa, reducing conditions

Product Datasheets

You must select a language.

x

5896-S5

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5896-S5

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and Tween® 20.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS View Larger

Recombinant Human Semaphorin 5A Fc Chimera Protein (Catalog # 5896-S5) has a molecular weight (MW) of 287.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Semaphorin 5A

Semaphorin 5A (Sema5A, previously called SemaF) is a 140 kDa protein of the semaphorin family of axon guidance molecules (1-4). Class 5 semaphorins are type I transmembrane glycoproteins with an N-terminal Sema domain and multiple juxtamembrane type 1 thrombospondin (TSP) repeats within their extracellular domains (1‑3). Sema5A is expressed developmentally in oligodendrocytes, neuroepithelial cells surrounding retinal axons, the base of limb buds, the cardiac atrial septum and endocardial cushions, and the mesoderm surrounding cranial vessels (4-6). The human Sema5A cDNA encodes a 22 amino acid (aa) signal sequence, a 946 aa extracellular domain (ECD), a 22 aa transmembrane sequence and an 85 aa cytoplasmic portion. Within aa 23 - 765, which includes the sema domain and four of the seven TSP repeats, human Sema5A shares 93% aa identity with corresponding mouse, rat, and canine sequences. Semaphorins typically transduce signals through transmembrane plexins (1, 2). The sema domain of Sema5A binds plexin B3, triggering signaling via HGF R/c-Met (7). Both Sema5A and plexin B3 are expressed postnatally during differentiation and migration of central nervous system oligodendrocytes. However, plexin B3 is not significantly expressed prenatally and therefore unlikely to be the Sema5A receptor during development (7, 8). The Sema5A TSP repeats interact with either heparin sulfate or chondroitin sulfate proteoglycans (HSPG, CSPG) (9). HSPG interaction promotes attraction, while CSPG interaction promotes repulsion and is essential for axon fasciculation, independent of plexin B3 (9, 10). Sema5A mutations have been implicated in the genetic syndrome, cri-du-chat, while some polymorphisms may increase risk for neurodegenerative diseases such as Parkinson’s (3, 11). Sema5A expression may be upregulated in metastatic cancer cells and downregulated in autism (12, 13).

References
  1. Flannery, E. and M. Duman-Scheel (2009) Curr. Drug Targets 10:611.
  2. Zhou, Y. et al. (2008) Trends Biol. Sci. 33:161.
  3. Simmons, A.D. et al. (1998) Biochem. Biophys. Res. Commun. 242:685.
  4. Oster, S.F. et al. (2003) Development 130:775.
  5. Goldberg, J.L. et al. (2004) J. Neurosci. 24:4989.
  6. Fiore, R. et al. (2005) Mol. Cell. Biol. 25:2310.
  7. Artigiani, S. et al. (2004) EMBO Rep. 5:710.
  8. Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
  9. Kantor, D.B. et al. (2004) Neuron 44:961.
  10. Hilario, J.D. et al. (2008) Dev. Biol. 326:190.
  11. Lin, L. et al. (2009) Trends Neurosci. 32:142.
  12. Pan, G-Q. et al. (2009) World J. Gastroenterol. 15:2800.
  13. Melin, M. et al. (2006) Neuropsychobiology 54:64.
Entrez Gene IDs
9037 (Human); 20356 (Mouse); 310207 (Rat)
Alternate Names
FLJ12815; sema domain, seven thrombospondin repeats (type 1 and type 1-like); Sema F; SEMA5A; SEMAF; Semaphorin 5A; semaphorin F; semaphorin-5A; Semaphorin-F; semF; transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A

Product Specific Notices

Tween is a registered trademark of ICI Americas.

Citations for Recombinant Human Semaphorin 5A Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. The Expression of IL-17, in Chronic Spontaneous Urticaria Is Linked to Semaphorin5A
    Authors: M Lobna, E Nasren, S Adi, B Jacob, GA Maria, M Marcus, V Zahava
    Biomolecules, 2021-03-02;11(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The association of semaphorin 5A with lymph node metastasis and adverse prognosis in cervical cancer
    Authors: JB Xiao, XL Li, L Liu, G Wang, SN Hao, HJ Dong, XM Wang, YF Zhang, HD Liu
    Cancer Cell Int., 2018-06-22;18(0):87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Loading...

Reviews for Recombinant Human Semaphorin 5A Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Semaphorin 5A Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Human Semaphorin 5A Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review