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Recombinant Human sFRP-2 Protein

Catalog #: 6838-FR
9 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
6838-FR-025/CF

With Carrier

Catalog # Availability Size / Price Qty
6838-FR-025
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Citations (9)
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Recombinant Human sFRP-2 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to synergize with Wnt-3a to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 10-40 ng/mL in the presence of 20 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Source
Mouse myeloma cell line, NS0-derived human sFRP-2 protein
Leu25-Cys295
Accession #
AAH08666
N-terminal Sequence
Analysis
Leu25 (major) & Phe26 (minor)
Predicted Molecular Mass
31 kDa
SDS-PAGE
34-36 kDa, reducing conditions

Product Datasheets

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6838-FR (with carrier)

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6838-FR/CF (carrier free)

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COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6838-FR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6838-FR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: sFRP-2

Secreted Frizzled Related Protein‑2 (sFRP‑2) belongs to a family of Wnt‑binding proteins with homology to the ligand‑binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30‑35 kDa in size and contain an N-terminal Frizzled‑like domain with 10 conserved cysteines and a Netrin‑like C‑terminal domain (1, 2). Mature human sFRP‑2, also known as SARP‑1, SDF‑5, and FRP‑2, shares 99% aa sequence identity with mouse and rat sFRP‑2 (3). sFRP‑2 is widely expressed during embryogenesis and in the adult tissues including the eye, heart, lung, colon, intestine, smooth muscle, pancreas, prostate, testis, kidney, brain, teeth and joints, craniofacial mesenchyme, and preadipocytes (3‑6). Depending on the context, sFRP‑2 can exert either positive or negative effects on Wnt signaling (7‑9). It also inhibits BMP‑induced effects (8, 10). sFRP‑2 can be incorporated into the extracellular matrix through interactions with Fibronectin and Integrin alpha 5 beta 1 (11). sFRP‑2 plays a variety of roles during tissue morphogenesis including inhibition of the planar cell polarity pathway and myoblast and osteoblast differentiation (8, 10, 12, 13). sFRP‑2 is also expressed in multiple myeloma and glioma in which it promotes tumorigenicity (10, 14). At physiological concentrations sFRP‑2 enhances BMP‑1 mediated proteolysis of Pro‑Collagen I, whereas at higher concentrations it inhibits BMP‑1 activity (15, 16). This difference is significant considering that sFRP‑2 is up‑regulated in fibrotic areas of the heart following myocardial infarction (15, 16). Elevated levels of sFRP‑2 then promote the recovery of cardiac function by reducing collagen deposition, remodeling, and calcification and by promoting the engraftment of mesenchymal stem cells into the heart (8, 15).

References
  1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Hu, E. et al. (1998) Biochem. Biophys. Res. Commun. 247:287.
  4. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
  5. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
  6. Leimeister, C. et al. (1998) Mech. Dev. 75:29.
  7. Galli, L.M. et al. (2006) Dev. Dyn. 235:681.
  8. Alfaro, M.P. et al. (2010) J. Biol. Chem. 285:35645.
  9. von Marschall, Z. and L.W. Fisher (2010) Biochem. Biophys. Res. Commun. 400:299.
  10. Oshima, T. et al. (2005) Blood 106:3160.
  11. Lee, J.-L. et al. (2004) J. Biol. Chem. 279:14602.
  12. Sugiyama, Y. et al. (2010) Dev. Biol. 338:193.
  13. Descamps, S. et al. (2008) Cell Tissue Res. 332:299.
  14. Roth, W. et al. (2000) Oncogene 19:4210.
  15. He, W. et al. (2010) Proc. Natl. Acad. Sci. 107:21110.
  16. Kobayashi, K. et al. (2009) Nat. Cell Biol. 11:46.
Long Name
Secreted Frizzled Related Protein 2
Entrez Gene IDs
6423 (Human); 20319 (Mouse)
Alternate Names
FRP2; FRP-2secreted apoptosis related protein 1; SARP-1; SARP1sFRP-2; SDF-5; Secreted apoptosis-related protein 1; secreted frizzled-related protein 2; sFRP2; sFRP-2

Citations for Recombinant Human sFRP-2 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. The role of dexamethasone in mediating the contradictory effects of Wnt antagonists SFRP2 and SFRP3 on human hair follicle growth
    Authors: Hamida, OB;Kim, MK;Kwack, MH;
    Scientific reports
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  2. A fibroblast-dependent TGF?1/sFRP2 noncanonical Wnt signaling axis underlies epithelial metaplasia in idiopathic pulmonary fibrosis
    Authors: Cohen, ML;Brumwell, AN;Ho, TC;Montas, G;Golden, JA;Jones, KD;Wolters, PJ;Wei, Y;Chapman, HA;Le Saux, CJ;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2
    Authors: YC Hsueh, RE Pratt, VJ Dzau, CP Hodgkinson
    Scientific Reports, 2023-03-09;13(1):3920.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Nitric oxide suppression by secreted frizzled-related protein 2 drives retinoblastoma
    Authors: P Jayabal, F Zhou, X Ma, KM Bondra, B Blackman, ST Weintraub, Y Chen, P Chévez-Bar, PJ Houghton, B Gallie, Y Shiio
    Cell Reports, 2023-02-10;42(2):112103.
    Species: N/A
    Sample Types: Phage
    Applications: Bioassay
  5. Langerhans cells and SFRP2/Wnt/beta-catenin signalling control adaptation of skin epidermis to mechanical stretching
    Authors: JK Ledwon, EE Vaca, CC Huang, LJ Kelsey, JL McGrath, J Topczewski, AK Gosain, JM Topczewska
    Journal of Cellular and Molecular Medicine, 2022-01-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Daam1 activates RhoA to regulate Wnt5a?induced glioblastoma cell invasion
    Authors: G Liu, T Yan, X Li, J Sun, B Zhang, H Wang, Y Zhu
    Oncol. Rep., 2017-12-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. PI3K? isoform-dependent activation of RhoA regulates Wnt5a-induced osteosarcoma cell migration
    Authors: A Zhang, T Yan, K Wang, Z Huang, J Liu
    Cancer Cell Int., 2017-02-14;17(0):27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Secreted Frizzled-Related Protein 2 (sFRP2) Functions as a Melanogenic Stimulator; the Role of sFRP2 in UV-Induced Hyperpigmentary Disorders.
    Authors: Kim M, Han J, Kim J, Park T, Kang H
    J Invest Dermatol, 2016-01-01;136(1):236-44.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  9. Commercially Available Preparations of Recombinant Wnt3a Contain Non-Wnt Related Activities Which May Activate TGF-beta Signaling.
    Authors: Carthy J, Engstrom U, Heldin C, Moustakas A
    J Cell Biochem, 2015-09-22;117(4):938-45.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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