Recombinant Human sFRP-3 Protein Summary
Product Specifications
Optimal concentrations should be determined by each laboratory for each application.
Ala33-Asn325, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
192-SF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
192-SF/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: sFRP-3
Secreted Frizzled Related Protein 3 (sFRP-3) was originally identified in bovine cartilage for its chondrogenic ability. Human, mouse, chick and Xenopus clones have also been isolated. SFRP-3 is often referred to as FRZB, other names include Fritz, Frzb1, and FRP-3. At the amino acid sequence level, sFRP-3 is highly conserved. The human protein shares 77% identity with Xenopus, 92% with mouse, and 94% with bovine proteins. Human sFRP-3 is strongly expressed in the developing appendicular skeleton, and cartilage of craniofacial bones. As determined by Northern blot of adult tissues, it is strongly detected in heart and placenta, as well as the brain, skeletal muscle, kidney and pancreas.
The N-terminal portion of the protein shows 50% amino acid identity to the corresponding region of the Drosophila frizzled gene product, a receptor for Wg/Wnt signals. The similarity of sFRP-3 with frizzled proteins is restricted to the N-terminal cysteine-rich domain (CRD) that contains at least ten cysteine residues with highly conserved spacing between them. SFRP-3 was subsequently shown to be a soluble antagonist of Wnt signals. It lacks all transmembrane domains of frizzled proteins but retains the ability to bind Wnts. Ectopic expression of sFRP-3 mRNA has been shown to interfere with the induction of secondary axes in Xenopus embryos injected with Xwnt-8 mRNA.
- Hoang, et al. (1996) J. Biol. Chem. 271:26131.
- Leyns, et al. (1997) Cell 88:747.
- Wang, et al. (1997) Cell 88:757.
- Mayr, et al. (1997) Mech. Dev. 63:109.
- Rattner, et al. (1997) PNAS 94:2859.
Citations for Recombinant Human sFRP-3 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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ECHO, the executable CHOndrocyte: A computational model to study articular chondrocytes in healthy and disease
Authors: S Schivo, S Khurana, K Govindaraj, J Scholma, J Kerkhofs, L Zhong, X Huang, J van de Pol, R Langerak, AJ van Wijnen, L Geris, M Karperien, JN Post
Cell. Signal., 2019-12-11;0(0):109471.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Reduced skeletal muscle secreted frizzled-related protein 3 is associated with inflammation and insulin resistance
Authors: AS Pachori, M Madan, YO Nunez Lope, F Yi, C Meyer, AA Seyhan
Obesity (Silver Spring), 2017-02-27;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Methylation and loss of secreted frizzled-related protein 3 enhances melanoma cell migration and invasion.
Authors: Ekstrom EJ, Sherwood V, Andersson T
PLoS ONE, 2011-04-08;6(4):e18674.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Production of Wnt inhibitors by myeloma cells: potential effects on canonical Wnt pathway in the bone microenvironment.
Authors: Giuliani N, Morandi F, Tagliaferri S, Lazzaretti M, Donofrio G, Bonomini S, Sala R, Mangoni M, Rizzoli V
Cancer Res., 2007-08-15;67(16):7665-74.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Frizzled-related protein variants are risk factors for hip osteoarthritis.
Authors: Lane NE, Lian K, Nevitt MC, Zmuda JM, Lui L, Li J, Wang J, Fontecha M, Umblas N, Rosenbach M, de Leon P, Corr M
Arthritis Rheum., 2006-04-01;54(4):1246-54.
Applications: ELISA (Standard)
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