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Recombinant Human Sonic Hedgehog/Shh Protein, High Activity

Catalog #: 8908-SH
11 Citations 1 Review Datasheet / COA / SDS
C-terminal cholesterol, N-terminal fatty acid-modified

Carrier Free

Catalog # Availability Size / Price Qty
8908-SH-005/CF

With Carrier

Catalog # Availability Size / Price Qty
8908-SH-005
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Enhanced Activity of Human Cell-expressed Sonic Hedgehog (Shh) Protein.
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Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Sonic Hedgehog/Shh Protein, High Activity Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is typically 6-36 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human Sonic Hedgehog/Shh protein
Cys24-Gly197
Accession #
Q15465
N-terminal Sequence
Analysis
Cys24
Structure / Form
Cholesterol-modified at the C-terminal and fatty acid-modified at the N-terminal
Predicted Molecular Mass
20 kDa
SDS-PAGE
18-24 kDa, reducing conditions

Product Datasheets

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8908-SH (with carrier)

Product Datasheet
COA
SDS

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8908-SH/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8908-SH

Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl and CHAPS with BSA as a carrier protein.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

8908-SH/CF

Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl and CHAPS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Bioactivity Enhanced Activity of Human Cell-expressed Sonic Hedgehog (Shh) Protein. View Larger

Recombinant Human Shh proteins induce alkaline phosphatase production by mesenchymal stem cells. High Activity Shh (green), purified from HEK293 cells and containing the correct post-translational modifications (cholesterol and fatty acids), is over 14-fold more active than E. coli-purified Recombinant Human Shh-N (C24II) N-Terminus (Catalog # 1845-SH; red line), and over 250-fold more active than E. coli-purified Recombinant Human Shh-N (Catalog # 1314-SH; blue line).

SDS-PAGE Enhanced Activity of Human Cell-expressed Sonic Hedgehog (Shh) Protein. View Larger

1 μg/lane of Recombinant Human Sonic Hedgehog/Shh Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing 18-24 kDa bands.

Mass Spectrometry Post-translational Modification Analysis of Naturally-modified Recombinant Human Sonic Hedgehog (Shh) Protein. View Larger

LC/ESI-MS analysis of Recombinant Human (rh)SHH Protein, High Activity shows major peaks at 20119.3, 20145.2, and 20171.6 Da, suggesting that recombinant human SHH molecules are dual-modified with cholesterol at C-terminus, and fatty acids (lauric acid, myristic acid, and palmitic acid) at the N-terminus. The minor peaks at 19776 Da corresponds to rhSHH with only fatty acid modification.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sonic Hedgehog/Shh

The Sonic Hedgehog (Shh) protein is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. The Shh protein regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue, Shh is associated with cancer development and tissue remodeling following injury (1-3). Human Shh encodes a 462 amino acid (aa) precursor Shh protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). The Shh-C protein, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). The Shh-N protein is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of the Shh protein as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase the receptor binding affinity and signaling potency of the Shh-N protein (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of functionality of the Shh protein and are required for the development of Shh protein gradients important in tissue morphogenesis (9, 13). Canonical signaling by the Shh protein is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). Binding of the Shh protein to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).

References
  1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
  2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
  3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
  4. Marigo, V. et al. (1995) Genomics 28:44.
  5. Zeng, X. et al. (2001) Nature 411:716.
  6. Feng, J. et al. (2004) Development 131:4357.
  7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
  8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
  9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
  10. Etheridge, L.A. et al. (2010) Development 137:133.
  11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
  12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
  13. Lewis, P.M. et al. (2001) Cell 105:599.
  14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
  15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
  16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.
Entrez Gene IDs
6469 (Human); 20423 (Mouse)
Alternate Names
HHG1; HHG-1; HLP3; HPE3; MCOPCB5; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; ShhNC; SMMCI; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS

Citations for Recombinant Human Sonic Hedgehog/Shh Protein, High Activity

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Diffusion barriers imposed by tissue topology shape Hedgehog morphogen gradients
    Authors: Schlissel, G;Meziane, M;Narducci, D;Hansen, AS;Li, P;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Increasing Ciliary ARL13B Expression Drives Active and Inhibitor-Resistant Smoothened and GLI into Glioma Primary Cilia
    Authors: Shi, P;Tian, J;Mallinger, JC;Ling, D;Deleyrolle, LP;McIntyre, JC;Caspary, T;Breunig, JJ;Sarkisian, MR;
    Cells
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Structural insights into proteolytic activation of the human Dispatched1 transporter for Hedgehog morphogen release
    Authors: W Li, L Wang, BM Wierbowski, M Lu, F Dong, W Liu, S Li, P Wang, A Salic, X Gong
    Nature Communications, 2021-11-29;12(1):6966.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. Forebrain Shh overexpression improves cognitive function and locomotor hyperactivity in an aneuploid mouse model of Down syndrome and its euploid littermates
    Authors: FJ Gao, D Klinedinst, FX Fernandez, B Cheng, A Savonenko, B Devenney, Y Li, D Wu, MG Pomper, RH Reeves
    Acta neuropathologica communications, 2021-08-16;9(1):137.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts
    Authors: J Sun, DY Shin, M Eiseman, AR Yallowitz, N Li, S Lalani, Z Li, M Cung, S Bok, S Debnath, SJ Marquez, TE White, AG Khan, IC Lorenz, JH Shim, FS Lee, R Xu, MB Greenblatt
    Nature Communications, 2021-07-29;12(1):4611.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. PAUPAR and PAX6 sequentially regulate human embryonic stem cell cortical differentiation
    Authors: Y Xu, J Xi, G Wang, Z Guo, Q Sun, C Lu, L Ma, Y Wu, W Jia, S Zhu, X Guo, S Bian, J Kang
    Nucleic Acids Research, 2021-02-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. GLI2 but not GLI1/GLI3 plays a central role in the induction of malignant phenotype of gallbladder cancer
    Authors: S Ichimiya, H Onishi, S Nagao, S Koga, K Sakihama, K Nakayama, A Fujimura, Y Oyama, A Imaizumi, Y Oda, M Nakamura
    Oncology reports, 2021-01-22;45(3):997-1010.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Nicotinamide restricts neural precursor proliferation to enhance catecholaminergic neuronal subtype differentiation from mouse embryonic stem cells
    Authors: SM Griffin, MR Pickard, CP Hawkins, AC Williams, RA Fricker
    PLoS ONE, 2020-09-14;15(9):e0233477.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Defects in mRNA Translation in LRRK2-Mutant hiPSC-Derived Dopaminergic Neurons Lead to Dysregulated Calcium Homeostasis
    Authors: JW Kim, X Yin, A Jhaldiyal, MR Khan, I Martin, Z Xie, T Perez-Rose, M Kumar, L Abalde-Atr, J Xu, L Chen, SM Eacker, DJ Surmeier, NT Ingolia, TM Dawson, VL Dawson
    Cell Stem Cell, 2020-08-25;27(4):633-645.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Structures of human Patched and its complex with native palmitoylated sonic hedgehog
    Authors: X Qi, P Schmiege, E Coutavas, J Wang, X Li
    Nature, 2018-07-11;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  11. Roles of Ihh signaling in chondroprogenitor function in postnatal condylar cartilage
    Authors: N Kurio, C Saunders, TE Bechtold, I Salhab, HD Nah, S Sinha, PC Billings, M Pacifici, E Koyama
    Matrix Biol., 2018-02-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human Sonic Hedgehog/Shh Protein, High Activity
By Boubacar Barry on 08/11/2017
Application: Binding assay/Protein-protein interaction
Reason for Rating: n/a