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Recombinant Human SSc5D His-tag Protein, CF

Catalog #: 10535-SS Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10535-SS-050

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Recombinant Human SSc5D His-tag Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human SSc5D His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles. The ED50 for this effect is 0.2-1 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human SSc5D protein
Val17-Thr875, with a C-terminal 6-His tag
Accession #
NP_001138422.1
N-terminal Sequence
Analysis
Val17
Predicted Molecular Mass
92 kDa
SDS-PAGE
135-153 kDa, under reducing conditions

Product Datasheets

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10535-SS

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10535-SS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human SSc5D His-tag Protein Binding Activity View Larger

Recombinant Human SSc5D His-tag (Catalog # 10535-SS) binds fluorescein-conjugated E. coli bioparticles. The ED50 for this effect is 0.2-1 μg/mL.

SDS-PAGE Recombinant Human SSc5D His-tag Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human SSc5D His-tag Protein (10535-SS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands 120-155 at kDa.

Reconstitution Calculator

Background: SSc5D

SSc5D (Soluble Scavenger with 5 Domains) is a member of scavenger receptor cysteine-rich (SRCR) superfamily that comprises a group of proteins with one or more domains sharing structural homology with the membrane distal extracellular domain of the macrophage type I scavenger receptor (1). Mature human SSc5D consists of 1573 amino acids (aa), including five SRCR domains at the N-terminus and a heavily glycosylated, mucin-like domain at the C-terminus. Within N‑terminal SRCR domains, human SSc5D shares 95% and 96% aa sequence identity with mouse and rat SSc5D, respectively (1). SSc5D is produced by monocyte/macrophages, T cells and several epithelial cells from placenta, spleen, and colon (1). Many members of the SRCR super family bind to and clear bacteria, fungi or viruses from infected host (2). SSc5D physically interacts with whole bacteria cells especially to E. coli strains. These findings can help fight bacterial infections by discriminating between different bacteria strains and species (3).

References
  1. Goncalves, C.M. et al. (2009) Mol. Immunol. 46:2585.
  2. Martinez, V.G. et al. (2011) Pharmacol. Rev. 63:967.
  3. Bessa Pereira, C. et al. (2016) Front. Immunol. 7:416.
Long Name
Scavenger Receptor Cysteine Rich Family Member With 5 Domains
Entrez Gene IDs
284297 (Human)
Alternate Names
S5D-SRCRB; scavenger receptor cysteine rich domain containing (5 domains); scavenger receptor cysteine-rich glycoprotein; soluble scavenger protein with 5 SRCR domains; soluble scavenger receptor cysteine-rich domain-containing protein SSC5D; soluble scavenger with 5 domains; SSc5D

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