Recombinant Human TAFA3/FAM19A3 Protein, CF

Recombinant Human TAFA3/FAM19A3 Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: TAFA3/FAM19A3

TAFA3, also called FAM19A3, is a member of the FAM19/TAFA family of small secreted proteins (1). Human TAFA3 has a molecular weight of approximately 15 kDa. The mature protein is 103 amino acids (aa) in length and shares 93% aa sequence identity with the mouse and rat orthologs (1). Alternative exon splicing causes a reading frame shift at aa 89, creating a 169 aa isoform with an altered C-terminal region (1). TAFA3 is specifically expressed in the brain with the highest levels being detected in parietal cortex and basal ganglia (1). TAFA proteins display chemokine-like signatures and appear to be distantly related to CCL3/ MIP-1 alpha as they share a common CC motif (1). TAFA3 has been suggested to be involved in transmitting the photoperiodically-controlled Melatonin signal from the pars tuberalis (2). TAFA3 mRNA levels in the pars tuberalis vary between night and day and correspond to plasma Melatonin levels (2). Additionally, these day/night expression differences are not observed in mice that lack Melatonin type 1 receptors (2). Interestingly, in other brain regions, such as the hippocampus and the ventral posterior nucleus of the thalamus, TAFA3 expression does not appear to be mediated by Melatonin, suggesting expression of this protein is controlled by region-specific mechanisms (2).