Recombinant Human Testican 2/SPOCK2 Protein, CF Summary
Product Specifications
Glu23-Trp424, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2328-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Testican 2/SPOCK2
Testican 2 encoded by the SPOCK2 gene is a proteoglycan highly expressed in brain (1, 2). The human and mouse cDNAs predict 94% identity between the deduced amino acid sequences from the two species, indicating a conserved function (3, 4). Testican 2 contains a Ca2+-binding domain and a C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. However, these domains may not be involved directly in the inhibition of targeted proteases. For example, Testican 2 is not able to inhibit MMP-14 (MT1-MMP, a metalloprotease) activation of MMP-2 (5). The purified rhTestican 2 is not capable of inhibiting rhMMP-14 and rhCathepsin L (R&D Systems, Catalog # 918-MP and 952-CY) in assays using the fluorogenic peptide substrates (R&D Systems, Catalog # ES001 and ES008). Nevertheless, over expression of Testican 2 is able to abrogate inhibition of MMP-14 by other testican family members and to inhibit neurite outgrowth (6, 7).
- Nagase, T. et al. (1996) DNA Res. 3:321.
- Vannahme, C. et al. (1999) J. Neurochem. 73:12.
- Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
- Okazaki, Y. et al. (2002) Nature 420:563.
- Nakada, M. et al. (2001) Cancer Res. 61:8896.
- Nakada, M. et al. (2003) Cancer Res. 63:3364.
- Hartmann, U. and P. Maurer (2001) Matrix Biol. 20:23.
Citation for Recombinant Human Testican 2/SPOCK2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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SPOCK2 modulates neuropathic pain by interacting with MT1-MMP to regulate astrocytic MMP-2 activation in rats with chronic constriction injury
Authors: Wang, C;Xu, Y;Xu, M;Sun, C;Zhang, X;Tao, X;Song, T;
Journal of neuroinflammation
Species: Rat
Sample Types: In Vivo
Applications: In vivo assay
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