Recombinant Human TfR2 Fc Chimera Protein, CF Summary
Product Specifications
| MD | Human IgG1 (Pro100-Lys330) | IEGR | Human TfR2 (Gly112-Phe801) Accession # NP_003218.2 |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10486-TF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Recombinant Human TfR2 Fc Chimera Protein (Catalog # 10486-TF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-117 kDa and 180-210 kDa, respectively.
Reconstitution Calculator
Background: TfR2
Transferrin Receptor 2 (TfR2 or TfR2 alpha) is an iron-binding type 2 transmembrane glycoprotein homologous to the Transferrin Receptor 1 (TfR or TfR1) (1). It forms a 215 kDa disulfide-linked homodimer and is predominantly expressed in the liver and modulates hepcidin production in response to iron (1, 2). Human TfR2 cDNA encodes 801 amino acids (aa) including an 83 aa N-terminal intracellular region, a 21 aa transmembrane domain, and a 697 aa extracellular domain (ECD). Human TfR2 ECD shares 86% and 45% aa identity with mouse TfR2 and human TfR1, respectively. An alternative splicing isoform (TfR2 beta) lacks the intracellular region, transmembrane domain and part of the extracellular regions and is expressed ubiquitously (1, 3). The Iron loaded Transferrin (holo-Transferrin) circulating in blood binds to cell-surface receptor TFR2 (4). Mutations in TFR2 can cause a form of hereditary hemochromatosis (HH, type 111), a disorder marked by chronic iron overload (5).
- Kawabata, H. et al. (1999) J. Biol. Chem. 274:20826.
- Trinder, D. and E. Baker (2003) Int. J. Biochem. Cell. Biol. 35:292.
- Deaglio, S. et al. (2002) Blood 100:3782.
- Aisen, P. et al. (1978) J. Biol Chem. 253:1930.
- Camaschella, C. et al. (2000) Nat Genet. 25:14.
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Reason for Rating: The protein delivered is either highly aggregated or high glycosylated. No evidence of function observed or provided.
