Recombinant Human Tie-2 His-tag Protein, CF

Catalog #: 10457-TI Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10457-TI-050

Recombinant Human Tie-2 His-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Tie-2 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Tie-2 His-tag (Catalog # 10457-TI) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 His-tag (Catalog # 623-AN) binds with an ED₅₀ of 0.5-4 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human Tie-2 protein
Ala23-Lys745, with a C-terminal 6-His tag

Accession #

AAA61139.1

N-terminal Sequence
Analysis

Ala23

Predicted Molecular Mass

81 kDa

SDS-PAGE

98-111 kDa, under reducing conditions

Product Datasheets

10457-TI

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10457-TI

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Tie-2 His-tag (10457-TI) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Human Angiopoietin-2 (623-AN) binds with an ED₅₀ of 0.5-4 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human Tie-2 His-tag Protein (Catalog # 10457-TI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 98-111 kDa.

Reconstitution Calculator

Background: Tie-2

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human Tie-2 cDNA encodes a 1124 amino acid (aa) precursor protein with an 18 aa putative signal peptide, a 727 aa extracellular domain (ECD) and a 354 aa cytoplasmic domain. Human Tie-2 ECD shares 90% aa identity with mouse and rat Tie-2. Two ligands, angiopoietin1 (Ang1) and angiopoietin2 (Ang2), which bind Tie-2 with high affinity, have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to over-express Ang2 or to lack Ang1 or Tie-2 display similar angiogenesis defects.

References

Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
Takakura, N. et al. (1998) Immunity 9:677.
Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.

Long Name

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2

Entrez Gene IDs

7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)

Alternate Names

angiopoietin-1 receptor; CD202b antigen; CD202b; EC 2.7.10; EC 2.7.10.1; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TEK tyrosine kinase, endothelial; TEK; Tie2; Tie-2; TIE2CD202b; Tunica interna endothelial cell kinase; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; venous malformations, multiple cutaneous and mucosal; VMCM; VMCM1