Recombinant Human TIM-1/KIM-1/HAVCR Fc Chimera Protein, CF
Recombinant Human TIM-1/KIM-1/HAVCR Fc Chimera Protein, CF Summary
Product Specifications
| Human Tim-1/Kim-1/HAVCR (Ser21-Thr288) Accession # AAC39862 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9319-TM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Human TIM‑1/KIM‑1/HAVCR Fc Chimera (Catalog # 9319-TM) inhibits anti-CD3-induced proliferation of PHA-activated human T cells. The ED50 for this effect is 0.6-3.6 μg/mL.
Reconstitution Calculator
Background: TIM-1/KIM-1/HAVCR
T cell immunoglobulin and mucin domain 1 (TIM-1), also known as KIM-1 and HAVcr1, is a member of the TIM family which is involved in the regulation of innate and adaptive immune responses (1). TIM-1 is a type I transmembrane protein that contains an N-terminal immunoglobulin-like domain, a mucin domain with O- and N-linked carbohydrates, a transmembrane segment, and a cytoplasmic signaling domain (2). Multiple TIM-1 variants can be produced due to polymorphisms or alternative splicing resulting in deletions in the mucin domain. Within the extracellular domain, human TIM-1 shares 41% aa sequence identity with mouse and rat TIM-1. TIM-1 is expressed on splenic B cells, IL-10+ regulatory B cells, CD4+ T cells, mast cells, invariant NKT (iNKT) cells, dendritic cells, kidney epithelium and a broad range of mucosal epithelium (1, 3-5). It is upregulated on activated Th2 cells, after dendritic cell maturation, and on kidney tubular epithelial cells after injury (6-9). Metalloproteinase-mediated cleavage of TIM-1 at the membrane-proximal region results in the release of a soluble form of TIM-1 which is detectable in the urine and in circulation (10). TIM-1 serves as a receptor for phosphatidylserine, LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses (2, 11-16). Its interaction with phosphatidylserine enables TIM-1 to mediate the phagocytosis of apoptotic cells (12, 13) and iNKT cell activation (17). TIM-1 binding induces the activation of LMIR5-expressing myeloid cells, contributing to tissue homeostasis as well as damage following kidney injury (14). TIM-1 ligation co-stimulates T cell activation and enhances Th2 cytokine production (7, 15). In humans, TIM-1 serves as a cellular entry receptor for various viruses, including hepatitis A virus, Ebolavirus and Marburgvirus (2, 11).
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- Ma, J. et al. (2011) Biochem. Biophys. Res. Commun. 406:223.
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- Kuehn, E.W. et al. (2002) Am. J. Physiol. Renal Physiol. 283:F1326.
- Umetsu, S.E. et al. (2005) Nat. Immunol. 6:447.
- Xiao, S. et al. (2011) Eur. J. Immunol. 41:1539.
- Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
- Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
- Kondratowicz, A.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:8426.
- Miyanishi, M. et al. (2007) Nature 450:435.
- Kobayashi, N. et al. (2007) Immunity 27:927.
- Yamanishi, Y. et al. (2010) J. Exp. Med. 207:1501.
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- Lee, H.H. et al. (2010) J. Immunol. 185:5225.
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