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Recombinant Human TINAGL1 Protein, CF

Catalog #: 9304-TU Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9304-TU-050
Recombinant Human TINAGL1 Protein Bioactivity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TINAGL1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Cultrex Mouse Laminin I (Catalog # 3400-010-01) is coated at 5 µg/mL, Recombinant Human TINAGL1 binds with an ED50 = 1-6 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human TINAGL1 protein
Human TINAGL1
(Ala22-Met464)
Accession # Q9GZM7		HPGGGSGGGSGGGSHHHHHH
Accession #
Q9GZM7
N-terminal Sequence
Analysis
Ala22
Predicted Molecular Mass
52 kDa
SDS-PAGE
47-61 kDa, reducing conditions

Product Datasheets

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9304-TU

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9304-TU

Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Brij-35.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Bioactivity Recombinant Human TINAGL1 Protein Bioactivity View Larger

When Cultrex Mouse Laminin I (Catalog # 3400-010-01) is coated at 5 µg/mL, Recombinant Human TINAGL1 (Catalog # 9304-TU) binds with an ED50 = 1-6 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TINAGL1

TINAGL1 (Tubulointerstitial nephritis antigen-like 1; also TIN-ag-RP, AZ-1 and Lipocalin 7) is a 54-57 kDa glycoprotein member of the peptidase C1 family of molecules. Mature human TINAGL1 contains an SMB (somatomedin B) domain, one vWFC domain, and a nonenzymatic peptidase C1A region (1). Mature human TINAGL1 shares 90% amino acid (aa) identity with mouse TINAGL1. Alternative splicing generates additional isoforms that either lack a portion of the vWFC domain or consist of a portion of the peptidase domain with a 38 aa insertion and flanked by substitutions (2). TINAGL1 is secreted by renal tubule epithelium, zona glomerulosa cells, vascular smooth muscle, and striated muscle cells (1, 3, 4). It is also expressed in mouse decidual endometrium and blastocysts (5-7). During pregnancy it binds to Laminin-1 and Integrins alpha 5 and beta 1 and plays a role in supporting fertility (6-8). In the vasculature, TINAGL1 localizes to basal laminae along adrenocortical sinusoidal capillaries where it binds to fibronectin and collagen and supports adrenocortical cell adhesion (9).

References
  1. Wex, T. et al. (2001) Biochemistry 40:1350.
  2. Zhou, B. et al. (2000) J. Am. Soc. Nephrol. 11:658.
  3. Bromme, N.C. et al. (2000) Biochem. Biophys. Res. Commun. 271:474.
  4. Mukai, K. et al. (2003) J. Biol. Chem. 278:17084.
  5. Sakurai, M. et al. (2014) Zygote 22:259.
  6. Tajiri, Y. et al. (2010) Biol. Reprod. 82:263.
  7. Igarashi, T. et al. (2009) Biol. Reprod. 81:948.
  8. Takahashi, A. et al. (2016) J. Reprod. Dev. 62:43.
  9. Li, D. et al. (2007) FEBS J. 274:2506.
Long Name
Tubulointerstitial Nephritis Antigen-like 1
Entrez Gene IDs
64129 (Human); 94242 (Mouse); 94174 (Rat)
Alternate Names
androgen-regulated gene 1; ARG1; GIS5; Glucocorticoid-inducible protein 5; LCN7; LIECG3; lipocalin 7; OLRG2; OLRG-2; Oxidized LDL-responsive gene 2 protein; oxidized-LDL responsive gene 2; P3ECSL; TIN Ag-related protein; TINAGL; TINAGL1; TINAG-like 1; TINAGRP; TIN-ag-RP; tubulointerstitial nephritis antigen-like 1; tubulointerstitial nephritis antigen-like; Tubulointerstitial nephritis antigen-related protein

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