Recombinant Human TREM-1 Fc Chimera Avi-tag Protein, CF
Recombinant Human TREM-1 Fc Chimera Avi-tag Protein, CF Summary
Learn more about Avi-tag Biotinylated ProteinsProduct Specifications
| Human TREM-1 (Ala21-Arg200) Accession # Q9NP99.1 | IEGRMD | Human IgG1 (Pro100-Lys330) | Avi-tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
AVI1278
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Measured by its binding ability in a functional ELISA. When Recombinant Human PGLYRP1/PGRP-S Protein (2590-PGB) is immobilized at 1 μg/mL (100 μL well), Biotinylated Recombinant Human TREM‑1 Fc Chimera Avi-tag Protein (Catalog # AVI1278) binds with an ED50 of 15.0‑150 ng/mL.
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2 μg/lane of Biotinylated Recombinant Human TREM‑1 Fc Chimera Avi-tag Protein (Catalog # AVI1278) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-78 kDa and 110-150 kDa, respectively.
Reconstitution Calculator
Background: TREM-1
TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1 and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS‑induced shock. Blockade of TREM-1 with a TREM‑1/Fc fusion protein protected mice against LPS‑induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1 - 3). Our Avi-tag Biotinylated human TREM-1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
- Bouchon, A. (2000) J. Immunol. 164:4991.
- Bouchon, A. (2001) Nature 410:1103.
- Nathan, C. and A. Ding (2001) Nature Med. 7:530.
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