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Recombinant Human TREML2/TLT-2 Fc Chimera Protein, CF

Catalog #: 3259-TL
3 Citations Datasheet / COA / SDS
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3259-TL-050
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TREML2/TLT-2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human PBMC cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with anti-CD3 and recombinant human TREML2/Fc Chimera.
The ED50 for this effect is 0.45-1.8 μg/mL.
Optimal dilutions should be determined by each laboratory for each applications.
Source
Mouse myeloma cell line, NS0-derived human TREML2/TLT-2 protein
Human TREML2
(Gly78 - Ser327)
Accession # CAI19909		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
CAI19909
N-terminal Sequence
Analysis
Gly78
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
53.7 kDa (monomer)
SDS-PAGE
75-100 kDa, reducing conditions

Product Datasheets

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3259-TL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3259-TL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TREML2/TLT-2

Trem-like transcript 2 protein (TLT-2; also TREML2) is a 33 kDa (unglycosylated) type I transmembrane cell surface receptor and member of the Trem family of receptor proteins. Human TLT-2 is synthesized as a 321 amino acid (aa) precursor that contains an 18 aa signal sequence, a 250 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 32 aa cytoplasmic tail (SwissProt # Q5T2D2). Within the ECD are an Ig-like V-type domain (aa 20 ‑ 121) and one potential site of N-linked glycosylation. Mature human TLT-2 shares 49% aa sequence identity with mature mouse TLT-2. Cell surface expression of TLT-2 is seen for most of the B and myeloid cell lines (1 ‑ 2). TLT-2 is also expressed constitutively on CD8+ T cells and induced on CD4+ T cells after activation (2). TLT-2 plays a role in the innate and adaptive immune responses. In 2008, Hashiguchi et al. discovered that the B7 family member B7-H3, which also plays important roles in immune responses, specifically bound to TLT-2. According to their research, stimulation with B7-H3 transfectants preferentially up-regulated the proliferation and IFN-gamma production on CD8+ T cells (2). Also, transduction of TLT-2 into T cells resulted in enhanced IL-2 and IFN-gamma production via interactions with B7-H3, and blockade of the B7‑H3:TLT‑2 pathway with mAb against B7-H3 or TLT-2 efficiently inhibited contact hypersensitivity responses (2). In 2009, however, Leitner et al. extensively analyzed interaction of B7-H3 with TLT-2 and found no evidence for such an interaction (3).

References
  1. King, R.G. et al. (2006) J. Immunol. 176:6012.
  2. Hashiguchi, M. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:10495.
  3. Leitner, J. et al. (2009) Eur. J. Immunol. 39:1754.
Long Name
Triggering Receptor Expressed on Myeloid Cells-like 2
Entrez Gene IDs
79865 (Human); 328833 (Mouse)
Alternate Names
C6orf76; dJ238O23.1; FLJ13693; MGC149715; MGC149716; TLT2; TLT-2; TREML2; trem-like transcript 2 protein; triggering receptor expressed on myeloid cells-like 2; triggering receptor expressed on myeloid cells-like protein 2; UNQ6268/PRO20473

Citations for Recombinant Human TREML2/TLT-2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity
    Authors: J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang
    Nature Communications, 2022-03-25;13(1):1606.
    Species: Human
    Sample Types: Transduced Whole Cells
    Applications: Flow Cytometry
  2. TIM-3 does not act as a receptor for galectin-9.
    Authors: Leitner, Judith, Rieger, Armin, Pickl, Winfried, Zlabinger, Gerhard, Grabmeier-Pfistershammer, Katharin, Steinberger, Peter
    PLoS Pathog, 2013-03-21;9(3):e1003253.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: Bioassay
  3. Identification of TLT2 as an engulfment receptor for apoptotic cells.
    Authors: de Freitas A, Banerjee S, Xie N
    J. Immunol., 2012-05-09;188(12):6381-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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