Recombinant Human TrkB His-tag Protein, CF Summary
Product Specifications
Cys32-His430, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
11549-TK
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Human TrkB His-tag Protein (Catalog # 11549-TK) binds Recombinant Human BDNF (BT-BDNF) with an ED50 of 1.50‑20.0 ng/mL.
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2 μg/lane of Recombinant Human TrkB His-tag Protein (Catalog # 11549-TK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74-82 kDa, under reducing conditions.
Reconstitution Calculator
Background: TrkB
The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.
- Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
- Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
- Soppet, D. et al. (1991) Cell 65:895.
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