Recombinant Human TROP-2 Fc Chimera Avi-tag Protein, CF

Catalog #: AVI11258 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI11258-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TROP-2 Fc Chimera Avi-tag Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IGF-II R/IGF2R (Catalog # 6418-GR) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human TROP-2 Fc Chimera Avi-tag (Catalog # AVI11258) binds with an ED₅₀ of 0.300-2.70 µg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human TROP-2 protein

Human TROP-2 (His27-Thr274) Accession # P09758.3	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

P09758.3

N-terminal Sequence
Analysis

No results obtained: His27 predicted by protein ID

Structure / Form

Disulfide-linked homodimer

Biotinylated via Avi-tag

Predicted Molecular Mass

56 kDa (monomer)

SDS-PAGE

65-80 kDa, under reducing conditions.

Product Datasheets

AVI11258

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11258

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human IGF-II R/IGF2R (6418-GR) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human TROP-2 Fc Chimera Avi-tag Protein (Catalog # AVI11258) binds with an ED₅₀ of 0.300-2.70 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human TROP-2 Fc Chimera Avi-tag Protein (Catalog # AVI11258) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-80 kDa and 120-160 kDa, respectively.

Reconstitution Calculator

Background:

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is transducing an intracellular calcium signal and may play a role in tumor growth. TROP-2 is associated with EGF mutation and resistance to drug treatment in many tumors. It has been discovered to bind to IGF IIR and promote IGF2-IGF1R-Akt (6). TROP-2 has emerged as a novel cancer target and a target for antibody-drug conjugates (7). TROP-2 interacts with ADAM-10 and binds E-Cadherin stimulating ADAM-10 mediated cleavage (8). Our Avi-tag Biotinylated human TROP-2 Fc chimera protein features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
Ripani, E. et al. (1998) Int. J. Cancer 76:671.
El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.
Sun, X. et al. (2021) J. Cancer 12:5310.
Goldenberg, D. M. et al. (2018) Oncotarget. 9:28989.
Guerra, E. et al. (2021) Neoplasia. 23:898.

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