Home / TROP-2 / Recombinant Human TROP-2 Fc Chimera Protein, CF

Recombinant Human TROP-2 Fc Chimera Protein, CF

Catalog #: 650-T2
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
650-T2-100
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Citations (4)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TROP-2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells or LS180 human colorectal adenocarcinoma cells. When 1 x 104 to 5 x 104 cells/well are added to Recombinant Human TROP‑2 Fc Chimera coated plates (10 µg/mL with 100 µL/well), >45% cells will adhere after 1 hour incubation.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human TROP-2 protein
Human TROP-2
(Thr88-Thr274)
Accession # P09758.3		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
P09758.3
N-terminal Sequence
Analysis
Thr88
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
49.2 kDa (monomer)
SDS-PAGE
70-80 kDa, reducing conditions

Product Datasheets

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650-T2

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

650-T2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TROP-2

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

References
  1. Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
  2. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
  3. Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
  4. Ripani, E. et al. (1998) Int. J. Cancer 76:671.
  5. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.
Long Name
Tumor-associated Calcium Signal Transducer 2
Entrez Gene IDs
4070 (Human); 56753 (Mouse)
Alternate Names
Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP-2; TROP2GA7331; tumor-associated calcium signal transducer 2

Citations for Recombinant Human TROP-2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Dual-inhibitory domain iCARs improve the efficiency of the AND-NOT gate CAR T strategy
    Authors: Bangayan, NJ;Wang, L;Burton Sojo, G;Noguchi, M;Cheng, D;Ta, L;Gunn, D;Mao, Z;Liu, S;Yin, Q;Riedinger, M;Li, K;Wu, AM;Stoyanova, T;Witte, ON;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Human
    Sample Types: Bacteriophage
    Applications: Bioassay
  2. The 2EF Antibody Targets a Unique N-Terminal Epitope of Trop-2 and Enhances the In Vivo Activity of the Cancer-Selective 2G10 Antibody
    Authors: Guerra, E;Trerotola, M;Relli, V;Lattanzio, R;Ceci, M;Boujnah, K;Pantalone, L;Di Pietro, R;Iezzi, M;Tinari, N;Alberti, S;
    Cancers
    Species: Human
    Sample Types:
    Applications: ELISA Capture
  3. Hybrid-Type SELEX for the Selection of Artificial Nucleic Acid Aptamers Exhibiting Cell Internalization Activity
    Authors: H Uemachi, Y Kasahara, K Tanaka, T Okuda, Y Yoneda, S Obika
    Pharmaceutics, 2021-06-15;13(6):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  4. TIM-3 does not act as a receptor for galectin-9.
    Authors: Leitner, Judith, Rieger, Armin, Pickl, Winfried, Zlabinger, Gerhard, Grabmeier-Pfistershammer, Katharin, Steinberger, Peter
    PLoS Pathog, 2013-03-21;9(3):e1003253.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: Bioassay

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