Recombinant Human TSLP Protein Summary
Product Specifications
Tyr29-Gln159, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1398-TS
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1398-TS/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
Recombinant Human TSLP (Catalog # 1398-TS) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with human IL-7 R alpha and human TSLP R. The ED50 for this effect is 0.05-0.3 ng/mL.
1 μg/lane of Recombinant Human TSLP was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Reconstitution Calculator
Background: TSLP
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells (1 - 3). The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to R gamma c.
- Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
- Park, L.S. et al. (2000) J. Exp. Med. 192:659.
- Pandey, A. et al. (2000) Nature Immunol. 1:59.
- Reche, P.A. et al. (2001) J. Immunol. 167:336.
- Quentmeier, H. et al. (2001) Leukemia 15:1286.
Citations for Recombinant Human TSLP Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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N-cadherin antagonism is bronchoprotective in severe asthma models
Authors: Pereira, NL;Schaible, N;Desai, A;Chan, EC;Ablooglu, AJ;Capuano, J;Lin, E;An, Z;Gebski, E;Jester, W;Ganesan, S;Balenga, N;Koziol-White, C;Panettieri, RA;Choudhury, S;Krishnan, R;Druey, KM;
Science advances
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay -
House dust mites stimulate thymic stromal lymphopoietin production in human bronchial epithelial cells and promote airway remodeling through activation of PAR2 and ERK signaling pathway
Authors: Hsieh, YA;Hsiao, YH;Ko, HK;Shen, YL;Huang, CW;Perng, DW;Su, KC;
Scientific reports
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Roseburia intestinalis stimulates TLR5-dependent intestinal immunity against Crohn's disease
Authors: Z Shen, W Luo, B Tan, K Nie, M Deng, S Wu, M Xiao, X Wu, X Meng, T Tong, C Zhang, K Ma, Y Liao, J Xu, X Wang
EBioMedicine, 2022-09-28;85(0):104285.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
RANKL down-regulates the mast cell proliferation through inducing senescence
Authors: JH Lim, HY Kim, HG Kang, HJ Jeong, HM Kim
Cytokine, 2022-08-30;159(0):156018.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Short isoform thymic stromal lymphopoietin reduces inflammation and aerobic glycolysis of asthmatic airway epithelium by antagonizing long isoform thymic stromal lymphopoietin
Authors: C Yu, W Huang, Z Zhou, S Liang, Z Zhou, J Liu, H Zhao, L Liu, H Dong, F Zou, S Cai
Respiratory Research, 2022-03-29;23(1):75.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IFN-gamma Induces PD-L1 Expression in Primed Human Basophils
Authors: SR Bonam, C Chauvin, MJ Mathew, J Bayry
Cells, 2022-02-25;11(5):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A multivariate modeling framework to quantify immune checkpoint context-dependent stimulation on T cells
Authors: L Karpf, C Trichot, L Faucheux, I Legbre, M Grandclaud, C Lahoute, H Mattoo, B Pasquier, V Soumelis
Cell Discovery, 2022-01-04;8(1):1.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Activated interleukin-7 receptor signaling drives B-cell acute lymphoblastic leukemia in mice
Authors: KR Thomas, EJ Allenspach, ND Camp, MN Wray-Dutra, S Khim, A Zielinska-, AE Timms, JP Loftus, HD Liggitt, K Georgopoul, SK Tasian, RG James, DJ Rawlings
Leukemia, 2021-06-30;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
p38 MAPK signalling regulates cytokine production in IL-33 stimulated Type 2 Innate Lymphoid cells
Authors: T Petrova, J Pesic, K Pardali, M Gaestel, JSC Arthur
Sci Rep, 2020-02-26;10(1):3479.
Species: Human
Sample Types: Cells
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Role of RANK-L as a potential inducer of ILC2-mediated type 2 inflammation in chronic rhinosinusitis with nasal polyps
Authors: N Ogasawara, JA Poposki, AI Klingler, BK Tan, KE Hulse, WW Stevens, AT Peters, LC Grammer, KC Welch, SS Smith, DB Conley, JR Raviv, P Soroosh, KI Takano, T Himi, RC Kern, RP Schleimer, A Kato
Mucosal Immunol, 2019-10-22;13(1):86-95.
Species: Human
Sample Types: Cells
Applications: Cell Culture -
Tumor-derived Thymic stromal lymphopoietin expands bone marrow B-cell precursors in circulation to support metastasis
Authors: E Ragonnaud, K Moritoh, M Bodogai, F Gusev, S Garaud, C Chen, X Wang, T Baljinnyam, KG Becker, RW Maul, K Willard-Ga, E Rogaev, A Biragyn
Cancer Res., 2019-10-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Structure-Activity Relationships of Baicalein and its Analogs as Novel TSLP Inhibitors
Authors: BB Park, JW Choi, D Park, D Choi, J Paek, HJ Kim, SY Son, AU Mushtaq, H Shin, SH Kim, Y Zhou, T Lim, JY Park, JY Baek, K Kim, H Kwon, SH Son, KY Chung, HJ Jeong, HM Kim, YW Jung, K Lee, KY Lee, Y Byun, YH Jeon
Sci Rep, 2019-06-19;9(1):8762.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Expression of c-Kit discriminates between two functionally distinct subsets of human type 2 innate lymphoid cells
Authors: T Hochdörfer, C Winkler, K Pardali, J Mjösberg
Eur. J. Immunol., 2019-03-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Estrogen restricts the apoptosis of endometrial stromal cells by promoting TSLP secretion
Authors: HL Yang, KK Chang, J Mei, WJ Zhou, LB Liu, L Yao, Y Meng, MY Wang, SY Ha, ZZ Lai, JF Ye, DJ Li, MQ Li
Mol Med Rep, 2018-08-24;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Application of a phenotypic drug discovery strategy to identify biological and chemical starting points for inhibition of TSLP production in lung epithelial cells
Authors: A Orellana, V García-Gon, R López, S Pascual-Gu, E Lozoya, J Díaz, D Casals, A Barrena, S Paris, M Andrés, V Segarra, D Vilella, R Malhotra, P Eastwood, A Planagumà, M Miralpeix, A Nueda
PLoS ONE, 2018-01-10;13(1):e0189247.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils
Authors: B Zhang, CY Wei, KK Chang, JJ Yu, WJ Zhou, HL Yang, J Shao, JJ Yu, MQ Li, F Xie
Oncol Lett, 2017-10-03;14(6):7483-7488.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Thymic stromal lymphopoietin-induced HOTAIR activation promotes endothelial cell proliferation and migration in atherosclerosis
Authors: Y Peng, K Meng, L Jiang, Y Zhong, Y Yang, Y Lan, Q Zeng, L Cheng
Biosci. Rep., 2017-07-12;37(4):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen
Authors: JT Schroeder, AP Bieneman
J. Immunol., 2017-06-26;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Effector and regulatory dendritic cells display distinct patterns of miRNA expression
Authors: V Lombardi, S Luce, H Moussu, L Morizur, C Gueguen, C Neukirch, S Chollet-Ma, L Mascarell, M Aubier, V Baron-Bodo, P Moingeon
Immun Inflamm Dis, 2017-05-12;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Group 2 innate lymphoid cells are elevated and activated in chronic rhinosinusitis with nasal polyps
Authors: JA Poposki, AI Klingler, BK Tan, P Soroosh, H Banie, G Lewis, KE Hulse, WW Stevens, AT Peters, LC Grammer, RP Schleimer, KC Welch, SS Smith, DB Conley, JR Raviv, JG Karras, O Akbari, RC Kern, A Kato
Immun Inflamm Dis, 2017-04-19;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-33, IL-25, and TSLP induce a distinct phenotypic and activation profile in human type 2 innate lymphoid cells
Authors: A Camelo, G Rosignoli, Y Ohne, RA Stewart, C Overed-Say, MA Sleeman, RD May
Blood Adv, 2017-03-30;1(10):577-589.
Species: Human
Sample Types: Whole Cells
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Whole-Genome Sequencing of a Family with Hereditary Pulmonary Alveolar Proteinosis Identifies a Rare Structural Variant Involving CSF2RA/CRLF2/IL3RA Gene Disruption
Authors: CY Chiu, SC Su, WL Fan, SH Lai, MH Tsai, SH Chen, KS Wong, WH Chung
Sci Rep, 2017-02-24;7(0):43469.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Distinct roles of short and long thymic stromal lymphopoietin isoforms in house dust mite-induced asthmatic airway epithelial barrier disruption
Sci Rep, 2016-12-20;6(0):39559.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Genetic, immunological, and clinical features of patients with bacterial and fungal infections due to inherited IL-17RA deficiency
Proc. Natl. Acad. Sci. U.S.A, 2016-12-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Myeloid dendritic cells exhibit defects in activation and function in patients with multiple sclerosis
Authors: Brigitte Wildemann
J. Neuroimmunol., 2016-11-02;301(0):53-60.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Thymic stromal chemokine TSLP acts through Th2 cytokine production to induce cutaneous T cell lymphoma
Cancer Res, 2016-09-06;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs
Authors: SM Bal, JH Bernink, M Nagasawa, J Groot, MM Shikhagaie, K Golebski, CM van Drunen, R Lutter, RE Jonkers, P Hombrink, M Bruchard, J Villaudy, JM Munneke, W Fokkens, JS Erjefält, H Spits, XR Ros
Nat Immunol, 2016-04-25;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
TSLP Is a Potential Initiator of Collagen Synthesis and an Activator of CXCR4/SDF-1 Axis in Keloid Pathogenesis.
Authors: Shin J, Kim S, Kim H, Noh J, Jin S, Park C, Lee W, Lee D, Lee J, Lee K
J Invest Dermatol, 2015-11-26;136(2):507-15.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Abnormal thymic stromal lymphopoietin expression in the duodenal mucosa of patients with coeliac disease
Authors: Gino R Corazza
Gut, 2015-09-04;65(10):1670-80.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Bioassay, ELISpot Development -
The Additive Inflammatory In Vivo and In Vitro Effects of IL-7 and TSLP in Arthritis Underscore the Therapeutic Rationale for Dual Blockade.
Authors: Hillen M, Hartgring S, Willis C, Radstake T, Hack C, Lafeber F, van Roon J
PLoS ONE, 2015-06-25;10(6):e0130830.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
Authors: Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova J, Puel A
J Exp Med, 2015-04-27;212(5):619-31.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Directional secretory response of double stranded RNA-induced thymic stromal lymphopoetin (TSLP) and CCL11/eotaxin-1 in human asthmatic airways.
Authors: Nino G, Huseni S, Perez G, Pancham K, Mubeen H, Abbasi A, Wang J, Eng S, Colberg-Poley A, Pillai D, Rose M
PLoS ONE, 2014-12-29;9(12):e115398.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
DeltaNp63 controls a TLR3-mediated mechanism that abundantly provides thymic stromal lymphopoietin in atopic dermatitis.
Authors: Kubo T, Kamekura R, Kumagai A, Kawata K, Yamashita K, Mitsuhashi Y, Kojima T, Sugimoto K, Yoneta A, Sumikawa Y, Yamashita T, Sato N, Himi T, Ichimiya S
PLoS ONE, 2014-08-29;9(8):e105498.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A thymic stromal lymphopoietin-responsive dendritic cell subset mediates allergic responses in the upper airway mucosa.
Authors: Melum G, Farkas L, Scheel C, Van Dieren B, Gran E, Liu Y, Johansen F, Jahnsen F, Baekkevold E
J Allergy Clin Immunol, 2014-06-21;134(3):613-621.e7.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand.
Authors: Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J
Nat Commun, 2014-06-09;5(0):4092.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Thymic stromal lymphopoietin links keratinocytes and dendritic cell-derived IL-23 in patients with psoriasis.
Authors: Volpe E, Pattarini L, Martinez-Cingolani C, Meller S, Donnadieu M, Bogiatzi S, Fernandez M, Touzot M, Bichet J, Reyal F, Paronetto M, Chiricozzi A, Chimenti S, Nasorri F, Cavani A, Kislat A, Homey B, Soumelis V
J Allergy Clin Immunol, 2014-06-06;134(2):373-81.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
NADPH oxidase-independent formation of extracellular DNA traps by basophils.
Authors: Morshed M, Hlushchuk R, Simon D, Walls A, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon H, Yousefi S
J Immunol, 2014-04-25;192(11):5314-23.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients.
Authors: Teunissen M, Munneke J, Bernink J, Spuls P, Res P, Te Velde A, Cheuk S, Brouwer M, Menting S, Eidsmo L, Spits H, Hazenberg M, Mjosberg J
J Invest Dermatol, 2014-03-21;134(9):2351-60.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Embryonic trophoblasts induce decidual regulatory T cell differentiation and maternal-fetal tolerance through thymic stromal lymphopoietin instructing dendritic cells.
Authors: Du M, Guo P, Piao H, Wang S, Sun C, Jin L, Tao Y, Li Y, Zhang D, Zhu R, Fu Q, Li D
J Immunol, 2014-01-22;192(4):1502-11.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
PLoS Biol, 2012-09-25;10(9):e1001395.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Macrophages participate in IL-17-mediated inflammation.
Authors: Barin JG, Baldeviano GC, Talor MV, Wu L, Ong S, Quader F, Chen P, Zheng D, Caturegli P, Rose NR, Cihakova D
Eur. J. Immunol., 2012-01-23;42(3):726-36.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
IL-25 in atopic dermatitis: a possible link between inflammation and skin barrier dysfunction?
Authors: Hvid M, Vestergaard C, Kemp K
J. Invest. Dermatol., 2010-09-23;131(1):150-7.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Thymic stromal lymphopoietin-induced human asthmatic airway epithelial cell proliferation through an IL-13-dependent pathway.
Authors: Semlali A, Jacques E, Koussih L, Gounni AS, Chakir J
J. Allergy Clin. Immunol., 2010-03-17;125(4):844-50.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
TSLP and downstream molecules in experimental mouse allergic conjunctivitis.
Authors: Zheng X, Ma P, De Paiva CS, Cunningham MA, Hwang CS, Pflugfelder SC, Li DQ
Invest. Ophthalmol. Vis. Sci., 2010-01-27;51(6):3076-82.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
FcepsilonRI-mediated thymic stromal lymphopoietin production by interleukin-4-primed human mast cells.
Authors: Okayama Y, Okumura S, Sagara H, Yuki K, Sasaki T, Watanabe N, Fueki M, Sugiyama K, Takeda K, Fukuda T, Saito H, Ra C
Eur. Respir. J., 2009-01-22;34(2):425-35.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
Int. Immunol., 2008-08-13;20(10):1321-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Human basophils activated by mast cell-derived IL-3 express retinaldehyde dehydrogenase-II and produce the immunoregulatory mediator retinoic acid.
Authors: Spiegl N, Didichenko S, McCaffery P, Langen H, Dahinden CA
Blood, 2008-05-21;112(9):3762-71.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation.
Authors: Seshasayee D, Lee WP, Zhou M, Shu J, Suto E, Zhang J, Diehl L, Austin CD, Meng YG, Tan M, Bullens SL, Seeber S, Fuentes ME, Labrijn AF, Graus YM, Miller LA, Schelegle ES, Hyde DM, Wu LC, Hymowitz SG, Martin F
J. Clin. Invest., 2007-12-01;117(12):3868-3878.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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We used this protein in an in-house ELISA along with mAb (1398) and pAb (AF1398) to quantify TSLP in human serum and plasma. This combination could not detect TSLP samples but generated a good standard curve.