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Recombinant Human TSPAN8-LEL Fc Chimera Protein, CF

Catalog #: 11550-TS Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
11550-TS-100
Recombinant Human TSPAN8-LEL Fc Chimera Protein SDS-PAGE.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TSPAN8-LEL Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Chinese Hamster Ovary cell line, CHO-derived human TSPAN8 protein
MDHuman IgG1
(Pro100-Lys330)
IEGRMHuman TSPAN8-LEL
(Lys110-Asn205)
Accession # P19075.1
N-terminusC-terminus
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
38 kDa
SDS-PAGE
40-50 kDa, under reducing conditions

Product Datasheets

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11550-TS

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11550-TS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE View Larger

2 μg/lane of Recombinant Human TSPAN8-LEL Fc Chimera Protein (Catalog # 11550-TS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-50 kDa and 80-100 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSPAN8

Tetraspanin 8, or TSPAN8, belongs to a superfamily of proteins that is characterized by four transmembrane domains, three intracellular domains and two extracellular loops: a small extracellular loop (SEL) and a large extracellular loop (LEL).   The extracellular loops form molecular webs that bring together cell surface proteins, facilitating the formation of stable and functional signalling complexes. Tetraspanins form microdomains on the plasma membrane that mediate diverse biological processes including adhesion, cell fusion, immune response, and tumor development (1-4).  Human TSPAN8 consists of 237 amino acids, with the LEL region spanning residues 110-205.  Within the LEL, human TSPAN8 shares 57% aa sequence identity with mouse and rat TSPAN8-LEL.

References
  1. Charrin, S. et al. (2014) J. Cell Sci. 127:3641.
  2. Yang, J. et al. (2024) Cell. 13:193.
  3. Hemler, M.E. (2005) Nat. Rev. Mol. Cell Biol.6:801.
  4. Kim, T-K. et al. (2015) Biochem. Biophys. Res. Commun. 468:774.
Long Name
Tetraspanin 8/Transmembrane 4 Superfamily, Member 3
Entrez Gene IDs
7103 (Human); 216350 (Mouse); 171048 (Rat)
Alternate Names
CO-029; tetraspanin 8; TM4SF3; TM4SF3tspan-8; Transmembrane 4 superfamily member 3tetraspanin-8; TSPAN8; Tspan-8; Tumor-associated antigen CO-029

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