Recombinant Human Ubiquitin, CF Summary
Product Specifications
ATVID | 10-His tag | SS | Ubiquitin (Met1 - Gly76) Accession # P62988 |
N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
701-UB
Formulation | Supplied as a 0.2 μm filtered solution in PBS. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Ubiquitin
Ubiquitin (Ub) is an 6-7 kDa polypeptide whose name derives from the observation that Ubiquitin possesses a highly conserved structure that is found in virtually all plant and animal species (1, 2). Ubiquitin is a globular 76 amino acids (aa) protein which contains multiple lysines and two C-terminal glycines. In humans, there are at least four genes that code for Ubiquitin. Found on human chromosomes 17 (UbB), 2 (UbA-80), 19 (UbA-52) and 12 (UbC), all genes code for a Ubiquitin polymer that undergoes proteolytic processing to generate free, monoubiquitin (3-7). In general, about one-half of all Ubiquitin exists in monomeric form within the cell (8). Ubiquitin can also be added posttranslationally to multiple cellular proteins. In conjunction with Ubiquitin ligases E1, E2 and E3, Ubiquitin is covalently attached to amino groups on target molecules via its C-terminal glycines, either at the N-terminus, or on any exposed amino acid that precedes the targets C-terminus (9). Further structural complexity may be added through Ubiquitin binding to Ubiquitin. Depending upon the exact pattern created, cellular proteins possessing UAD (Ub-associated domain) and UIM (Ub-interacting motif) sequences will selectively bind ubiquitinated proteins and incorporate them into multiple signaling pathways or regulatory complexes (10, 11).
-
Rechsteiner, M. (1987) Annu. Rev. Cell Biol. 3:1.
-
Hershko, A. and A. Ciechanover (1998) Annu. Rev. Biochem. 67:425.
-
Kim, N-S. et al. (1998) J. Biochem. 124:35.
-
Lund, P.K. et al. (1985) J. Biol. Chem. 260:7609.
-
Redman, K.L. and M. Rechsteiner (1989) Nature 338:438.
-
Monia, B.P. et al. (1989) J. Biol. Chem. 264:4093.
-
Wiborg, O. et al. (1985) EMBO J. 4:755.
-
Agell, N. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3693.
-
Ciechanover, A. and R. Ben-Saadon (2004) Trends Cell Biol. 14:103.
-
Kim, H. T. et al. (2007) J. Biol. Chem. 282:17375.
-
Ye, Y. and M. Rape (2009) Nat. Rev. Mol. Cell Biol. 10:755.
Citation for Recombinant Human Ubiquitin, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Extracellular Administration of BCL2 Protein Reduces Apoptosis and Improves Survival in a Murine Model of Sepsis.
Authors: Iwata A, de Claro RA, Morgan-Stevenson VL
PLoS ONE, 2011-02-24;6(2):e14729.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human Ubiquitin, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Ubiquitin, CF and earn rewards!
Have you used Recombinant Human Ubiquitin, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image