Recombinant Human URB Protein, CF

Catalog #: 3410-UR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
3410-UR-050

R&D Systems Recombinant Proteins and Enzymes

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Recombinant Human URB Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit proliferation of HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 1-4 µg/mL.

Source

Mouse myeloma cell line, NS0-derived human URB protein
Met129-Tyr950 & Ser79-Tyr950, both with C-terminal 6-His tag

Accession #

Q76M96

N-terminal Sequence
Analysis

Met129 & Ser79

Predicted Molecular Mass

100.1 kDa

SDS-PAGE

125-150 kDa, reducing conditions

Product Datasheets

3410-UR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3410-UR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: URB

URB (upregulated in BRS-3 deficient mice) is a 150 kDa, secreted glycoprotein that belongs to the sushi-repeat-containing protein superfamily (1, 2). Sushi repeats, otherwise known as short consensus repeats (SCRs), are 60 amino acid (aa) sequences usually involved in protein-protein interaction. They are characterized by the presence of four Cys, two Pro, one Gly and one Trp (3). Human URB is synthesized as a 950 aa precursor that contains a 21 aa signal sequence and a 929 aa mature region. The mature molecule contains three extended sushi/SCR domains of approximately 140 aa each. They bear resemblance to the fifth sushi-repeat in human SPRX (4). The three lie between aa 141 - 281, 615 - 760, and 771 - 913, respectively. Between the first and second SCR lie two amino acid-rich regions, a Thr-rich domain (aa 347 - 404), and a Lys-rich domain (aa 487 - 588). Three potential N-linked glycosylation sites exist in the last two SCR’s, while six potential bipartite nuclear localization signals (NLS) occur between aa 420 - 780. There are two potential alternate splice forms for human URB. One is 594 aa in length, and shows a simple truncation at Ser594. This effectively removes the second and third SCRs and two bipartite NLS (5). The second is 553 aa in length and shows a simple truncation after Lys553. This eliminates four bipartite NLSs, the second and third SCRs, and part of the Lys-rich domain (6). Full-length human URB is 83%, 84% and 87% aa identical to rat, mouse and bovine URB, respectively. URB is found in chondrocytes and appears to be downregulated upon CFU-Fibroblast differentiation (1). Thus, it may play a role in skeletogenesis.

References