Home / VSIG10L / Recombinant Human VSIG10L Fc Chimera Protein, CF

Recombinant Human VSIG10L Fc Chimera Protein, CF

Catalog #: 9600-VS Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9600-VS-050
Recombinant Human VSIG10L Fc Chimera Protein Bioactivity
1 Image
Product Details
FAQs
Supplemental Products
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VSIG10L Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 1-6 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human VSIG10L protein
Human VSIG10L
(Leu28-Ala776)
Accession # Q86VR7-1 		IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
Q86VR7-1
N-terminal Sequence
Analysis
Leu28 and Ser176
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
106 kDa
SDS-PAGE
120-140 kDa, reducing conditions

Product Datasheets

You must select a language.

x

9600-VS

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9600-VS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human VSIG10L Fc Chimera Protein Bioactivity View Larger

Recombinant Human VSIG10L Fc Chimera (Catalog # 9600-VS) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 1-6 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: VSIG10L

V-set and immunoglobulin domain-containing protein 10-like (VSIG-10L) is an 840 amino acid (aa) single-pass type I membrane protein of the Ig superfamily. The protein consists of a 749 aa extracellular domain (ECD), a 21 aa helical transmembrane domain, and a 70 aa cytoplasmic domain. The ECD of VSIG-10L contains both an Ig-like C2-type 1 domain and an Ig-like C2-type 2 domain. The ECD of mature human VSIG-10L shares 95%, 75%, and 74% identity with Rhesus macaque, mouse, and rat, respectively. The specific biological function of VSIG-10L has yet to be elucidated, but there is evidence to suggest the molecule is involved in cell‑cell adhesion. Ig-like C2-type domains are similar to antibody constant domains and can be found in T cell adhesion molecules, such as Cluster of Differentiation 2 (CD2) along with vascular and intercellular cell adhesion molecules (VCAM and ICAM, respectively) (1). VSIG-10L is highly expressed in healthy esophagus with squamous mucosa (2). Down‑regulation of the protein may be linked to familial Barrett's esophagus and esophageal adenocarcinoma (2) while up‑regulation may be linked to lung squamous cell carcinoma (3). R&D in house data indicate that VSIG-10L inhibits the secretion of IFN-g from anti-CD3 activated human PBMCs.

References
  1. Yang H and Reinherz E (2001) J. Biol. Chem. 276(22):18775.
  2. Fecteau RE, et al. (2016) JAMA Oncol. 2(10):1333.
  3. Tian F, et al. (2017) J. Thorac. Dis. 9(1):42.
Long Name
V-Set and Immunoglobulin Domain Containing 10 Like
Entrez Gene IDs
147645 (Human); 75690 (Mouse); 308556 (Rat)
Alternate Names
V-Set And Immunoglobulin Domain Containing 10 Like; V-Set And Immunoglobulin Domain-Containing Protein 10-Like; VSIG10L

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Loading...

Reviews for Recombinant Human VSIG10L Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human VSIG10L Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Human VSIG10L Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review