Recombinant Human Wnt-16b Protein Summary
Product Specifications
Asn30-Lys365
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7790-WN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
7790-WN/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Wnt-16b
Wnt‑16 is a 40 kDa protein within the Wnt family of secreted, highly conserved, cysteine‑rich, palmitoylated cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Wnt‑16a and Wnt‑16b isoforms in humans differ in the signal sequence and the first two amino acids (aa) of the mature protein (2, 3). Wnt‑16b is the more conserved isoform and is widely expressed, while Wnt‑16a is expressed mainly in the human pancreas (3). Mature human Wnt‑16b shares 92%, 93%, and 95% aa sequence identity with mouse/rat, rabbit/porcine/equine, and bovine Wnt‑16, respectively. Wnt‑16 expression is detected on uterine stroma adjacent to the luminal epithelium during implantation (4). It is up‑regulated during the first embryonic lymphoid progenitor differentiation (5). Congenital heart defects correlate with elevated Wnt‑16 in mouse embryos and human amniotic fluid (6). Low cortical bone thickness and bone mineral density correlate with deletion of Wnt‑16 in mice and a Wnt‑16 missense SNP in humans (7). Wnt‑16 is over‑expressed in cells undergoing replicative senescence, and is up‑regulated in articular cartilage by injury and osteoarthritis (8, 9). Wnt‑16b expression in skin is up‑regulated in human basal cell carcinomas, enhancing cell survival (10). Its expression is also up‑regulated by DNA damage (radiation and chemotherapy) in stroma surrounding prostate tumors, causing enhanced survival and treatment resistance in the tumor cells (11). Pre‑B acute lymphoblastic leukemia with t(1;19) translocation, creating an E2A‑Pbx1 fusion protein, also causes up‑regulation of Wnt‑16 that confers resistance to apoptosis (12, 13). Wnt‑16 signaling through both canonical and JNK‑mediated (non‑canonical) pathways is reported (8‑10).
- Clevers, H. and R. Nusse (2012) Cell 149:1192.
- Katoh, H. and M. Katoh 2005) Oncol. Rep. 13:771.
- Fear, M.W. et al. (2000) Biochem. Biophys. Res. Commun. 278:814.
- Hayashi, K. et al. (2009) Biol. Reprod. 80:989.
- Corrigan, P.M. et al. (2009) Stem Cells Dev. 18:759.
- Nath, A.K. et al. (2009) PLoS ONE 4:e4221.
- Zheng, H.F. et al. (2012) PLoS Genet. 8:31002745.
- Dell’accio, F. et al. (2008) Arthritis Rheum. 58:1410.
- Binet, R. et al. (2009) Cancer Res. 69:9183.
- Teh, M.T. et al. (2006) J. Cell Sci. 120:330.
- Sun, Y. et al. (2012) Nat. Med. 18:1359.
- McWhirter, J.R. et al. (1999) Proc. Natl. Acad. Sci. USA 96:11464.
- Mazieres, J. et al. (2005) Oncogene 24:5396.
Citations for Recombinant Human Wnt-16b Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Wnt5a and ROR1 activate non-canonical Wnt signaling via RhoA in TCF3-PBX1 acute lymphoblastic leukemia and highlight new treatment strategies via Bcl-2 co-targeting
Authors: H Karvonen, R Perttilä, W Niininen, V Hautanen, H Barker, A Murumägi, CA Heckman, D Ungureanu
Oncogene, 2019-01-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation
Authors: R Diacou, Y Zhao, D Zheng, A Cvekl, W Liu
Cell Rep, 2018-11-27;25(9):2510-2523.e4.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism
Authors: LY Hung, D Sen, TK Oniskey, J Katzen, NA Cohen, AE Vaughan, W Nieves, A Urisman, MF Beers, MF Krummel, DR Herbert
Mucosal Immunol, 2018-10-18;0(0):.
Species: Human
Sample Types: In Vivo
Applications: In Vivo -
Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity.
Authors: Kilander M, Dahlstrom J, Schulte G
Cell Signal, 2014-05-27;26(9):1943-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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