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Recombinant Human Wnt-5b Protein

Catalog #: 7347-WN
15 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
7347-WN-025/CF

With Carrier

Catalog # Availability Size / Price Qty
7347-WN-025
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Citations (15)
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Recombinant Human Wnt-5b Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 125-750 ng/mL, in the presence of 5 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human Wnt-5b protein
Gln18-Lys359
Accession #
NP_110402
N-terminal Sequence
Analysis
Gln18 predicted: No results obtained, sequencing might be blocked
Predicted Molecular Mass
38.5 kDa
SDS-PAGE
45 kDa, reducing conditions

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7347-WN (with carrier)

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SDS

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7347-WN/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7347-WN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7347-WN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-5b

Wnt proteins are cysteine‑rich secreted glycoproteins that play critical roles in both carcinogenesis and embryonic development. Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low‑density lipoprotein receptor‑related protein family (LRP‑5 or ‑6), or the Ryk atypical receptor tyrosine kinase (1‑3). Downstream effects of Wnt signaling occur through multiple pathways with differing intracellular components: the canonical Wnt/ beta ‑catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (PCP) pathway (1‑4). Wnt‑5b is a 49 kDa glycoprotein that is implicated in the Wnt/Ca2+ and PCP pathways (4‑8). These pathways can inhibit canonical Wnt/ beta ‑catenin signaling (4, 7). Human and mouse Wnt‑5b are synthesized as 359 amino acid (aa) precursors that contain a 17 aa signal sequence and a 342 aa mature region. Mature human Wnt‑5b shares 95%, 94%  90%, 89% and 86% aa identity with mature mouse, rat, bovine, chick and Xenopus Wnt‑5b, respectively. Although Wnt‑5a and Wnt‑5b share 83% aa identity, their effects may be different. For example, Wnt-5b, but not Wnt‑5a, promotes cell cycle progression and is weakly transforming (8, 9). Wnt‑5a and ‑5b are thought to coordinate developmental events, such as chondrocyte differentiation and formation of endochondral bone (5, 6, 10). In contrast to more focused expression of Wnt‑5a, Wnt‑5b is constitutively expressed at low levels throughout mouse embryonic development (10, 11). In adult mice, Wnt-5b is widely expressed, including heart, liver, brain, lung, testes, kidney, and pancreas (11‑13). Wnt‑5b is up‑regulated during early adipogenesis, and its overexpression in 3T3‑L1 cells partially inhibits canonical Wnt suppression of adipogenesis (7, 14). It enhances PPAR gamma expression and promotes differentiation of preadipocytes (14). Human Wnt‑5b polymorphisms have been associated with Type II diabetes (12).

References
  1. Mikels, A.J. & R. Nusse, 2006, Oncogene 25:7461.
  2. Miller, J.R. (2002) Genome Biol. 3:3001.
  3. Li, L et al. (2004) J. Neurosci 29:5873.
  4. Veeman, M.T. et al. (2003) Developmental Cell 5:367.
  5. Fazzi, R. et al. (2011) PLoS ONE 6:e25600.
  6. Bradley, E.W. and M.H. Drissi (2011) J. Cell. Physiol. 226:1683.
  7. Kanazawa, A. et al. (2005) Biochem. Biophys. Res. Com. 330:505.
  8. Wong, G.T. et al. (1994) Mol. Cell. Biol. 14:6278.
  9. Yang, Y. et al. (2003) Development 130:1003.
  10. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  11. Gavin, B. J. et al. (1990) Genes Dev. 4:2319.
  12. Kanazawa, A. et al. (2004) Am. J. Hum. Genet. 75:832.
  13. Heller, R.S. et al. (2002) Dev. Dyn. 225:260.
  14. van Tienen, F.H.J. et al. (2009) Biochem. Biophys. Res. Commun. 387:207.
Long Name
Wingless-type MMTV Integration Site Family, Member 5b
Entrez Gene IDs
81029 (Human); 22419 (Mouse)
Alternate Names
MGC2648; protein Wnt-5b; wingless-type MMTV integration site family, member 5B; WNT-5B protein; Wnt5b; Wnt-5b

Citations for Recombinant Human Wnt-5b Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Establishment of the Myeloid TBX-Code Reveals Aberrant Expression of T-Box Gene TBX1 in Chronic Myeloid Leukemia
    Authors: Nagel, S;Haake, J;Pommerenke, C;Meyer, C;MacLeod, RAF;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. DLL4-Notch3-WNT5B axis mediates bi-directional pro-metastatic crosstalk between melanoma and lymphatic endothelial cells
    Authors: Alve, S;Gramolelli, S;Jukonen, J;Juteau, S;Pink, A;Manninen, AA;Hänninen, S;Monto, E;Lackman, MH;Carpén, O;Saharinen, P;Karaman, S;Vaahtomeri, K;Ojala, PM;
    JCI insight
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. In�vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion
    Authors: S Gupta, R Kawaguchi, E Heinrichs, S Gallardo, S Castellano, I Mandric, BG Novitch, SJ Butler
    Oncogene, 2022-07-19;40(3):111119.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis
    Authors: CL Wu, A Dicks, N Steward, R Tang, DB Katz, YR Choi, F Guilak
    Nature Communications, 2021-01-13;12(1):362.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. WNT11-FZD7-DAAM1 signalling supports tumour initiating abilities and melanoma amoeboid invasion
    Authors: I Rodriguez-, O Maiques, L Kohlhammer, G Cantelli, A Perdrix-Ro, J Monger, B Fanshawe, VL Bridgeman, SN Karagianni, RM Penin, J Marcolval, RM Marti, X Matias-Gui, GO Fruhwirth, JL Orgaz, I Malanchi, V Sanz-Moren
    Nat Commun, 2020-10-20;11(1):5315.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Aberrant expression of NKL homeobox genes HMX2 and HMX3 interferes with cell differentiation in acute myeloid leukemia
    Authors: S Nagel, C Pommerenke, C Meyer, RAF MacLeod, HG Drexler
    PLoS ONE, 2020-10-13;15(10):e0240120.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  7. Divergent effects of Wnt5b on IL-3- and GM-CSF-induced myeloid differentiation
    Authors: MM de Rezende, JP Ng-Blichfe, GZ Justo, EJ Paredes-Ga, R Gosens
    Cell. Signal., 2019-12-17;67(0):109507.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial Progenitors
    Authors: X Wu, EM van Dijk, JP Ng-Blichfe, IST Bos, C Ciminieri, M Königshoff, LEM Kistemaker, R Gosens
    Cells, 2019-09-25;8(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Tumour cell-derived WNT5B modulates in vitro lymphangiogenesis via induction of partial endothelial-mesenchymal transition of lymphatic endothelial cells
    Oncogene, 2016-09-05;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: ELISA (Standard)
  10. Downregulation of the Canonical WNT Signaling Pathway by TGFbeta1 Inhibits Photoreceptor Differentiation of Adult Human Muller Glia with Stem Cell Characteristics.
    Authors: Angbohang A, Wu N, Charalambous T, Eastlake K, Lei Y, Kim Y, Sun X, Limb G
    Stem Cells Dev, 2015-11-12;25(1):1-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. A FOXM1 Dependent Mesenchymal-Epithelial Transition in Retinal Pigment Epithelium Cells.
    Authors: Choudhary P, Dodsworth B, Sidders B, Gutteridge A, Michaelides C, Duckworth J, Whiting P, Benn C
    PLoS ONE, 2015-06-29;10(6):e0130379.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Lymphatic vessels arise from specialized angioblasts within a venous niche.
    Authors: Nicenboim, J, Malkinson, G, Lupo, T, Asaf, L, Sela, Y, Mayseless, O, Gibbs-Bar, L, Senderovich, N, Hashimshony, T, Shin, M, Jerafi-Vider, A, Avraham-Davidi, I, Krupalnik, V, Hofi, R, Almog, G, Astin, J W, Golani, O, Ben-Dor, S, Crosier, P S, Herzog, W, Lawson, N D, Hanna, J H, Yanai, I, Yaniv, K
    Nature, 2015-06-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis.
    Authors: Keats E, Dominguez J, Grant M, Khan Z
    Stem Cells, 2014-06-01;32(6):1649-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity.
    Authors: Kilander M, Dahlstrom J, Schulte G
    Cell Signal, 2014-05-27;26(9):1943-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Wnt5b promotes the cell motility essential for metastasis of oral squamous cell carcinoma through active Cdc42 and RhoA.
    Authors: Takeshita A, Iwai S, Morita Y, Niki-Yonekawa A, Hamada M, Yura Y
    Int J Oncol, 2013-11-12;44(1):59-68.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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