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Recombinant Human Wnt-7a Protein

Catalog #: 3008-WN
19 Citations Datasheet / COA / SDS

Discontinued Product

3008-WN has been discontinued.
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Citations (19)
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Recombinant Human Wnt-7a Protein Summary

Product Specifications

Purity
>70%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 1-3 µg/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human Wnt-7a protein
Leu32-Lys349
Accession #
O00755
N-terminal Sequence
Analysis
Leu32
Predicted Molecular Mass
35.5 kDa
SDS-PAGE
40-65 kDa, reducing conditions

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3008-WN (with carrier)

Product Datasheet
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3008-WN/CF (carrier free)

Discontinued Product

3008-WN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Wnt-7a

Human Wnt-7a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to receptors of the Frizzled family in conjunction with low-density lipoprotein receptor-related proteins (LRPs). Downstream effects of Wnt signaling occur through activation of one of three different intracellular pathways: the canonical Wnt pathway, Wnt/Ca2+ pathway, and planar cell polarity. The highly transforming Wnts, including Wnt-1, -3, -3a, -7a and -8 activate the canonical pathway, which regulates beta ‑catenin-mediated gene expression (1, 2). Human Wnt-7a is a 48 kDa secreted glycoprotein containing 24 cysteine residues that is expressed by epithelial and epithelially-derived cells of the placenta, kidney, testis, uterus, fetal lung and fetal and adult brain (3, 4). Palmitate modification of a cysteine residue has been shown for Wnt-3a; this site is conserved on all Wnts and is Cys73 on Wnt7a (5). When modified, increased hydrophobicity and activity is expected. Human Wnt-7a shows 97% aa identity with mouse, rat and dog Wnt-7a and 92% aa identity with chicken Wnt-7a. During development, Wnt7a is expressed by the dorsal ectoderm and drives expression of homeodomain transcription factors that control effectors important in patterning and cell fates in adjacent mesenchyme (6 - 10). When Wnt-7a is deleted, mice show disruption of dorsalization and anterior/posterior patterning during limb development and abnormalities in the reproductive tract (6 - 10). Wnt-7a is frequently downregulated in leukemia and lung cancers, potentially affecting homeobox (HOX) gene expression, differentiation state and growth control (11, 12). Roles for Wnt-7a have also been shown during formation of neural synapses, response of the uterus to estrogen and inflammatory cartilage destruction (10, 13, 14).

References
  1. Logan, C. Y. and R. Nusse (2004) Annu. Rev. Cell Dev. Biol. 20:781.
  2. Wong, G. T. et al. (1994) Mol. Cell. Biol. 14:6278.
  3. http://www.stanford.edu/~rnusse/wntwindow.html 
  4. Bui, T. D. et al. (1997) Gene 189:25.
  5. Willert, K. et al. (2003) Nature 423:448.
  6. Parr, B. A. and A.P. McMahon (1995) Nature 374:350.
  7. Cygan, J. A. et al. (1997) Development 124:5021.
  8. Parr, B.A. and A.P. McMahon (1998) Nature 395:707.
  9. Miller, C. and D. Sassoon (1998) Development 125:3201.
  10. Carta, L. and D. Sassoon (2004) Biol. Reprod. 71:444.
  11. Calvo, R. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12776.
  12. Winn, R.A. et al. (2005) J. Biol Chem. 280:19625.
  13. Hall, A.C. et al. (2000) Cell 100:525.
  14. Hwang, S.-G. et al. (2004) J. Biol. Chem 279:26597.
Long Name
Wingless-type MMTV Integration Site Family, Member 7a
Entrez Gene IDs
7476 (Human)
Alternate Names
protein Wnt-7a; proto-oncogene Wnt7a protein; wingless-type MMTV integration site family, member 7A; Wnt7a; Wnt-7a

Citations for Recombinant Human Wnt-7a Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua
    Authors: VC Cheung, CY Peng, M Marini?, NJ Sakabe, I Aneas, VJ Lynch, C Ober, MA Nobrega, JA Kessler
    Cell Reports, 2021-05-18;35(7):109138.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Involvement of Wnt7a in the role of M2c microglia in neural stem cell oligodendrogenesis
    Authors: M Mecha, N Yanguas-Ca, A Feliú, L Mestre, FJ Carrillo-S, K Riecken, D Gomez-Nico, C Guaza
    J Neuroinflammation, 2020-03-19;17(1):88.
    Species: Human, Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. EGFR-Aurka Signaling Rescues Polarity and Regeneration Defects in Dystrophin-Deficient Muscle Stem Cells by Increasing Asymmetric Divisions
    Authors: YX Wang, P Feige, CE Brun, B Hekmatneja, NA Dumont, JM Renaud, S Faulkes, DE Guindon, MA Rudnicki
    Cell Stem Cell, 2019-01-31;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes
    Authors: SH Lee, SM Shin, P Zhong, HT Kim, DI Kim, JM Kim, W Do Heo, DW Kim, CY Yeo, CH Kim, QS Liu
    Nat Commun, 2018-08-24;9(1):3434.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Wnt7a induces satellite cell expansion, myofiber hyperplasia and hypertrophy in rat craniofacial muscle
    Authors: X Cheng, H Huang, X Luo, B Shi, J Li
    Sci Rep, 2018-07-13;8(1):10613.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  6. Tissue transglutaminase regulates interactions between ovarian cancer stem cells and the tumor niche
    Authors: S Condello, LE Sima, C Ivan, H Cardenas, GE Schiltz, RK Mishra, D Matei
    Cancer Res., 2018-03-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Morphological and molecular comparisons between tibialis anterior muscle and levator veli palatini muscle: A preliminary study on their augmentation potential
    Authors: X Cheng, L Song, M Lan, B Shi, J Li
    Exp Ther Med, 2017-10-30;15(1):247-253.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  8. WNT/?-catenin pathway modulates the TNF-?-induced inflammatory response in bronchial epithelial cells
    Authors: J Jang, Y Jung, S Chae, SI Chung, SM Kim, Y Yoon
    Biochem. Biophys. Res. Commun, 2017-01-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. LPS-induced inflammatory response is suppressed by Wnt inhibitors, Dickkopf-1 and LGK974
    Authors: J Jang, Y Jung, Y Kim, EH Jho, Y Yoon
    Sci Rep, 2017-01-27;7(0):41612.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Porcupine-dependent Wnt signaling controls stromal proliferation and endometrial gland maintenance through the action of distinct WNTs
    Authors: Omar Farah
    Dev. Biol, 2016-12-10;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways.
    Authors: Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, Hwang S, Renshaw P
    PLoS ONE, 2014-08-29;9(8):e104937.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity.
    Authors: Kilander M, Dahlstrom J, Schulte G
    Cell Signal, 2014-05-27;26(9):1943-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Wnt7a stimulates myogenic stem cell motility and engraftment resulting in improved muscle strength.
    Authors: Bentzinger C, von Maltzahn J, Dumont N, Stark D, Wang Y, Nhan K, Frenette J, Cornelison D, Rudnicki M
    J Cell Biol, 2014-04-07;205(1):97-111.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Noncanonical Wnt5a enhances Wnt/beta-catenin signaling during osteoblastogenesis.
    Authors: Okamoto M, Udagawa N, Uehara S, Maeda K, Yamashita T, Nakamichi Y, Kato H, Saito N, Minami Y, Takahashi N, Kobayashi Y
    Sci Rep, 2014-03-27;4(0):4493.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Activation of intracellular calcium by multiple Wnt ligands and translocation of beta-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/beta-catenin pathways.
    Authors: Thrasivoulou C, Millar M, Ahmed A
    J Biol Chem, 2013-10-24;288(50):35651-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Wnt7a treatment ameliorates muscular dystrophy
    Proc. Natl. Acad. Sci. U.S.A., 2012-11-26;109(0):20614-20619.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Bioassay, In Vivo
  17. Heparan sulfate 6-O-endosulfatases (Sulfs) coordinate the Wnt signaling pathways to regulate myoblast fusion during skeletal muscle regeneration.
    Authors: Tran, Thanh H, Shi, Xiaofeng, Zaia, Joseph, Ai, Xingbin
    J Biol Chem, 2012-08-03;287(39):32651-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus.
    Authors: Gogolla N, Galimberti I, Deguchi Y, Caroni P
    Neuron, 2009-05-28;62(4):510-25.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  19. Establishment of a transitory dorsal-biased window of localized Ca2+ signaling in the superficial epithelium following the mid-blastula transition in zebrafish embryos.
    Authors: Ma LH, Webb SE, Chan CM, Zhang J, Miller AL
    Dev. Biol., 2008-12-24;327(1):143-57.
    Species: Zebrafish
    Sample Types: Whole Cells
    Applications: Bioassay

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