Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF

Catalog #: 10973-HA Datasheet / COA / SDS

His-tag (HEK293-Expressed)

Catalog #	Availability	Size / Price	Qty
10973-HA-100

Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein Binding Activity.

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin-1 (Catalog # 1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H1N1 His-tag (Catalog # 10973-HA) that produces 50% of the optimal binding response is 0.8-8.0 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived influenza a virus h1n1 Hemagglutinin protein
Asp18-Gln529, with a C-terminal 6-His tag

Accession #

YP_009118626.1

N-terminal Sequence
Analysis

Asp18

Predicted Molecular Mass

58 kDa

SDS-PAGE

68-83 kDa, under reducing conditions.

Product Datasheets

10973-HA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10973-HA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Galectin-1 (1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein (Catalog # 10973-HA) that produces 50% of the optimal binding response is 0.800-8.00 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein (Catalog # 10973-HA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 68-83 kDa.

Reconstitution Calculator

Background: Hemagglutinin

Hemagglutinin (HA) and neuraminidase (NA) are major membrane glycoproteins found on the surface of influenza virus. The two proteins are essential for the infectious cycle of the influenza virus. During initial infection, HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. HA exists as a homotrimer, with each monomer composed of a globular HA1 domain and the stalk-like HA2 domain. HA has evolved to recognize long alpha 2-6 sialylated glycans for efficient transmission in human population. Although the HA sequence patterns of the 1918 pandemic and 2009 pandemic viruses are similar, they were caused by different H1N1 subtype viruses. The H1N1 1918 HA shares 86% amino acid sequence identity with the H1N1 2009 HA. Inhibiting HA-mediated membrane fusion is a target for influenza therapies. The HA section in the 1918 virus is being currently used to investigate how the 1918 pandemic spread and how a modern pandemic influenza virus would play out in the future.

References

Michal Lazniewski, M. et al. (2018) Briefings in Functional Genomics 17:415.
Sriwilaijaroen, N. and Suzuki, Y. (2012) Proc Jpn Acad Ser B Phys Biol Sci. 88:226.
Chang, Y.J. et al. (2021) Sci Rep 11:8637.
Short, K. et al. (2018) Front. Cell. Infect. Microbiol. 8:343.

Long Name

Hemagglutinin

Entrez Gene IDs

23308115 (Influenza A Virus H1N1)

Alternate Names

HA; Hemagglutinin