Recombinant Monkeypox Virus Zaire-96-I-16 E8L Protein, CF

Recombinant Monkeypox Virus Zaire-96-I-16 E8L Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background:

Monkeypox Virus (MPXV), the virus that causes monkeypox infection in both humans and animals, is a double-stranded DNA virus that has had a recent global outbreak in 2022 (1). MPXV belongs to the Poxviridae family of viruses (2). It consists of several key subunits including a surface membrane fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L ~14 kDa and H3L ~32kDa), an envelope glycoprotein, A35R ~15 kDa), a receptor glycoprotein that mimics IFN‑alpha/beta (B16, ~37kDa), a palmitoylated EEV membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L, ~14kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding protein (I1L, ~36kDa), and a subunit required for DNA packaging (L1R, 18 kDa) (2-3).The E8L subunit in MPXV is a surface binding protein that plays a key role in viral entry (4). This happens with E8L binding to chondroitin sulfate on the surface of cells, giving MPXV virion attachment to the host cell. In infected samples, E8L is identified in abundance with intracellular proteins (5).  When targeting the E8L subunit, transmission and replication was reduced by 78% when treated with 100 nM of small interference RNA (siRNA) and reduced by 95% when treated with 200 nM of siRNA (4). The E8L subunit is the most promising target for a newer, more effective vaccine against MPXV, as it has been found to contain human-foreign pentapeptides that could contain epitopes that would be viable for MPXV vaccinations (4, 6-7).