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Recombinant Mouse Axl Fc Chimera (CHO-expressed) Protein, CF

Catalog #: 7477-AX Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
7477-AX-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse Axl Fc Chimera (CHO-expressed) Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Axl Fc Chimera is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Gas6 (Catalog # 986‑GS) that produces 50% optimal binding response is 0.4‑2 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Axl protein
Mouse Axl
(His20-Pro443)
Accession # Q00993		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
Q00993
N-terminal Sequence
Analysis
His20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
73.6 kDa (monomer)
SDS-PAGE
90-110 kDa, reducing conditions

Product Datasheets

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7477-AX

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7477-AX

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Axl

Axl, also known as Ufo and Ark, is a widely expressed 140 kDa glycoprotein in the TAM receptor tyrosine kinase family. TAM family receptors (Dtk/Tyro3, Axl, and Mer) are involved in regulation of the inflammatory response, cell survival and migration, and tumorigenesis (1). Mature mouse Axl consists of a 427 aa extracellular domain (ECD) that contains two Ig-like domains and two fibronectin type III domains, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain that includes the tyrosine kinase domain (2, 3). Within the ECD, mouse Axl shares 81% and 95% aa sequence identity with human and rat Axl, respectively. Axl binds the vitamin K‑dependent protein Gas6 which triggers tyrosine autophosphorylation of the Axl cytoplasmic domain (4). Activation of Axl induces a broad range of activities including platelet aggregation and thrombus formation (5), macrophage and dendritic cell phagocytosis of apoptotic cells (6), NK cell development from hematopoietic progenitor cells (7), and in vivo angiogenesis (8). Axl is highly expressed in solid cancers and promotes in vivo tumorigenesis and tumor cell invasiveness (8, 9). It contributes to vascular remodeling and inflammatory cell infiltration in response to hypertension and restricted blood flow (10). It also functions as a cellular entry receptor for Gas6‑opsonized lentiviruses (11). A 70‑80 kDa soluble portion of the Axl ECD can be shed by proteolytic cleavage, and this fragment retains the ability to bind Gas6 (12, 13).

References
  1. Linger, R.M.A. et al. (2011) Adv. Cancer Res. 100:35.
  2. Faust, M. et al. (1992) Oncogene 7:1287.
  3. Rescigno, J. et al. (1991) Oncogene 6:1909.
  4. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  5. Cosemans, J.M.E.M. et al. (2010) J. Thromb. Haemost. 8:1797.
  6. Seitz, H.M. et al. (2007) J. Immunol. 178:5635.
  7. Park, I.-K. et al. (2009) Blood 113:2470.
  8. Holland, S. et al. (2005) Cancer Res. 65:9294.
  9. Rankin, E.B. et al. (2010) Cancer Res. 70:7570.
  10. Korshunov, V.A. et al. (2006) Circ. Res. 98:1446.
  11. Morizono, K. et al. (2011) Cell Host Microbe 9:286.
  12. O’Bryan, J.P. et al. (1995) J. Biol. Chem. 270:551.
  13. Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838.
    Long Name
    Axl Receptor Tyrosine Kinase
    Entrez Gene IDs
    558 (Human); 26362 (Mouse); 102126361 (Cynomolgus Monkey)
    Alternate Names
    AI323647; ARK; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; Tyro7; tyrosine-protein kinase receptor UFO; UFO; UFOoncogene AXL

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