Recombinant Mouse B7-1/CD80 Fc Chimera His-tag Protein, CF

Catalog #: 740-B1
10 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
740-B1-100
740-B1-01M

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Citations (10)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse B7-1/CD80 Fc Chimera His-tag Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED₅₀ for this effect is 5‑30 ng/mL in the presence of PHA.

Source

Mouse myeloma cell line, NS0-derived mouse B7-1/CD80 protein

Mouse B7-1 (Asp37-Lys245) Accession # Q00609	DIEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Accession #

Q00609

N-terminal Sequence
Analysis

Asp37

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

51.3 kDa (monomer)

SDS-PAGE

80-90 kDa, reducing conditions

Product Datasheets

740-B1

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

740-B1

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile, deionized water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: B7-1/CD80

B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses (1). Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response (2-6). Mature mouse B7-1 consists of a 209 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 22 aa transmembrane segment, and a 38 aa cytoplasmic domain (7). Within the ECD, mouse B7-1 shares 50% and 68% aa sequence identity with human and rat B7-1, respectively. Alternative splicing generates a short isoform that lacks the second Ig-like domain but retains the ability to bind CD28 (8). Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4 (1). B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells (2).

References

Ville, S. et al. (2015) Front. Immunol. 6:411.
Azuma, M. et al. (1993) Nature 366:76.
Freeman, G.J. et al. (1993) Science 262:909.
Chen, C. et al. (1994) J. Immunol. 152:4929.
Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
Lanier, L. et al. (1995) J. Immunol. 154:97.
Freeman, G. et al. (1991) J. Exp. Med. 174:625.
Inobe, M. et al. (1994) Biochem. Biophys. Res. Commun. 200:443.

Entrez Gene IDs

941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)

Alternate Names

Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80

Related Research Areas

Citations for Recombinant Mouse B7-1/CD80 Fc Chimera His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Aire suppresses CTLA-4 expression from the thymic stroma to control autoimmunity
Authors: J Morimoto, M Matsumoto, R Miyazawa, H Yoshida, K Tsuneyama, M Matsumoto
Cell Reports, 2022-02-15;38(7):110384.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Dual and Opposite Costimulatory Targeting with a Novel Human Fusion Recombinant Protein Effectively Prevents Renal Warm Ischemia Reperfusion Injury and Allograft Rejection in Murine Models
Authors: J Guiteras, L De Ramon, E Crespo, N Bolaños, S Barcelo-Ba, L Martinez-V, P Fontova, M Jarque, A Torija, O Bestard, D Resina, JM Grinyó, J Torras
International Journal of Molecular Sciences, 2021-01-26;22(3):.
Species: Rat
Sample Types: In Vivo
Applications: Surface Plasmon Resonance
IL-12 stimulates CTLs to secrete exosomes capable of activating bystander CD8(+) T cells
Authors: L Li, SM Jay, Y Wang, SW Wu, Z Xiao
Sci Rep, 2017-10-17;7(1):13365.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
CTLA-4 is expressed by activated mouse NK cells and inhibits NK Cell IFN-gamma production in response to mature dendritic cells.
Authors: Stojanovic A, Fiegler N, Brunner-Weinzierl M, Cerwenka A
J Immunol, 2014-03-31;192(9):4184-91.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Cytotoxic-T-lymphocyte antigen 4 receptor signaling for lymphocyte adhesion is mediated by C3G and Rap1.
Authors: Kloog, Yoel, Mor, Adam
Mol Cell Biol, 2014-01-06;34(6):978-88.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Soluble production of a biologically active single-chain antibody against murine PD-L1 in Escherichia coli.
Authors: Drees J, Augustin L, Mertensotto M, Schottel J, Leonard A, Saltzman D
Protein Expr Purif, 2013-11-08;94(0):60-6.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Antigen controls IL-7Ralpha expression levels on CD8 T cells during full activation or tolerance induction.
Authors: Hammerbeck CD, Mescher MF
J. Immunol., 2008-02-15;180(4):2107-16.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses.
Authors: Butte MJ, Keir ME, Phamduy TB, Sharpe AH, Freeman GJ
Immunity, 2007-07-12;27(1):111-22.
Applications: Bioassay
B7-1 and B7-2 selectively recruit CTLA-4 and CD28 to the immunological synapse.
Authors: Pentcheva-Hoang T, Egen JG, Wojnoonski K, Allison JP
Immunity, 2004-09-01;21(3):401-13.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor.
Authors: Shin T, Kennedy G, Gorski K, Tsuchiya H, Koseki H, Azuma M, Yagita H, Chen L, Powell J, Pardoll D, Housseau F
J. Exp. Med., 2003-07-07;198(1):31-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay