Recombinant Mouse B7-H3 Protein Summary
Product Specifications
Val29-Phe244, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1397-B3
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1397-B3/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: B7-H3
T cells require a signal induced by the engagement of the T cell receptor and a “costimulatory” signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with costimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3, and PD-L2 (1). B7-H3 is expressed at very high levels in immature dendritic cells at moderate levels on mature dendritic cells, LPS stimulated immature dendritic cells and LPS stimulated monocytes, and at low levels on resting monocytes. B7-H3 binds to activated T cells via an as-of-yet identified receptor. B7-H3 co-stimulates proliferation of T cells and interferon-gamma (IFN-gamma ) production and enhances the induction of cytotoxic T cells. B7-H3 shares 20 - 27% amino acid (aa) identity with other B7 family members (2). Murine B7-H3 is a 259 aa protein containing an extracellular domain, a transmembrane domain and a cytoplasmic domain. Mouse and human B7-H3 share 87% aa identity (3).
- Coyle, A.J. and J.-C. Gutierrez-Ramos (2001) Nature Immunol. 2:203.
- Chapoval, A.I. et al. (2001) Nature Immunol. 2:269.
- Sun, M. et al. (2002) J. Immunol. 168:6294.
Citations for Recombinant Mouse B7-H3 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Molecular imaging of T cell co-regulator factor B7-H3 with 89Zr-DS-5573a
Authors: IJG Burvenich, S Parakh, FT Lee, N Guo, Z Liu, HK Gan, A Rigopoulos, GJ O'Keefe, SJ Gong, YW Goh, H Tochon-Dan, FE Scott, M Kotsuma, K Hirotani, G Senaldi, AM Scott
Theranostics, 2018-07-30;8(15):4199-4209.
Species: Mouse
Sample Types: Recombinant Protein
Applications: ELISA (Capture) -
B7-H3 promoted proliferation of mouse spermatogonial stem cellsthe PI3K signaling pathway
Authors: X Wei, K Li, G Zhang, Y Huang, J Lv, M Li, L Zhao, C Fan, J Pu, J Hou, H Yuan
Oncotarget, 2017-12-20;9(2):1542-1552.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
B7-H3 Augments Inflammatory Responses and Exacerbates Brain Damage via Amplifying NF-?B p65 and MAPK p38 Activation during Experimental Pneumococcal Meningitis
Authors: X Chen, Y Li, S Blankson, M Liu, D Huang, HP Redmond, J Huang, JH Wang, J Wang
PLoS ONE, 2017-01-31;12(1):e0171146.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
B7-H3 participates in the development of Asthma by augmentation of the inflammatory response independent of TLR2 pathway
Authors: W Gu, X Zhang, Y Yan, Y Wang, L Huang, M Wang, X Shao, Z Chen, W Ji
Sci Rep, 2017-01-17;7(0):40398.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
B7H3 ameliorates LPS-induced acute lung injury via attenuation of neutrophil migration and infiltration
Sci Rep, 2016-08-12;6(0):31284.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Ultrasound molecular imaging in a human CD276 expression-modulated murine ovarian cancer model.
Authors: Lutz A, Bachawal S, Drescher C, Pysz M, Willmann J, Gambhir S
Clin Cancer Res, 2014-01-03;20(5):1313-22.
Applications: Western Blot -
B7-H3 participates in the development of experimental pneumococcal meningitis by augmentation of the inflammatory response via a TLR2-dependent mechanism.
Authors: Chen X, Quinn EM, Ni H, Wang J, Blankson S, Redmond HP, Wang JH, Feng X
J. Immunol., 2012-06-01;189(1):347-55.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
B7-h3 augments the inflammatory response and is associated with human sepsis.
Authors: Zhang G, Wang J, Kelly J
J. Immunol., 2010-08-09;185(6):3677-84.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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