Recombinant Mouse BMP-5 Protein Summary
Product Specifications
Ala315-His452
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6176-BM
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 200 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
6176-BM/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl. |
| Reconstitution | Reconstitute at 200 μg/mL in 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: BMP-5
Bone Morphogenetic Protein-5 (BMP-5) is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor beta (TGF-beta ) superfamily (1). BMP-5 is synthesized as a 452 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 20 kDa C-terminal mature protein (2, 3). Mature BMP-5 contains seven conserved cysteine residues involved in formation of the cysteine knot and the single interchain disulfide bond. Biologically active BMP-5 is a disulfide-linked homodimer of the C-terminal mature protein. Mature mouse BMP-5 shares 96% and 99% aa sequence identity with human and rat BMP-5, respectively. Cellular responses to BMP-5 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors (1). BMP-5 is expressed by chondrocytes in proliferating and hypertrophic zones of bone growth plates (4). It contributes to limb development by promoting proliferation and differentiation of chondrocytes as well as apoptosis of undifferentiated mesoderm (4, 5). Genetic defects in BMP-5 which cause C-terminal truncation or loss of the proteolytic cleavage site result in multiple skeletal abnormalities, including the short ear phenotype in mice (3, 6). BMP-5 is also expressed by ovarian granulosa cells where it functions as an autocrine factor to promote GC proliferation and inhibit their production of progesterone (7). In the nervous system, BMP-5 promotes dendrite outgrowth and dopaminergic neuronal differentiation (8, 9). It is upregulated in oral squamous carcinoma cells and induces the apoptosis of some myeloma cell lines (10, 11).
- Chen, D. et al. (2004) Growth Factors 22:233.
- Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.
- King, J.A. et al. (1994) Dev. Biol. 166:112.
- Mailhot, G. et al. (2008) J. Cell. Physiol. 214:56.
- Zuzarte-Luis, V. et al. (2004) Dev. Biol. 272:39.
- Ho, A.M. et al. (2008) BMC Dev. Biol. 8:35.
- Pierre, A. et al. (2005) Biol. Reprod. 73:1102.
- Beck, H.N. et al. (2001) BMC Neurosci. 2:12.
- Brederlau, A. et al. (2002) Mol. Cell. Neurosci. 21:367.
- Jin, Y. et al. (2001) Oral Oncol. 37:225.
- Ro, T.B. et al. (2004) Oncogene 23:3024.
Citations for Recombinant Mouse BMP-5 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Expression of Kielin/chordin-like protein is regulated by BMP-2 in osteoblasts
Authors: Toba, K;Yamada, A;Sasa, K;Shirota, T;Kamijo, R;
Bone reports
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Bone marrow-derived epithelial cells and hair follicle stem cells contribute to development of chronic cutaneous neoplasms
Authors: H Park, S Lad, K Boland, K Johnson, N Readio, G Jin, S Asfaha, KS Patterson, A Singh, X Yang, D Londono, A Singh, C Trempus, D Gordon, TC Wang, RJ Morris
Nat Commun, 2018-12-13;9(1):5293.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
BMP/SMAD pathway promotes neurogenesis of midbrain dopaminergic neurons in vivo and in human induced pluripotent and neural stem cells
Authors: VM Jovanovic, A Salti, H Tilleman, K Zega, MM Jukic, H Zou, RH Friedel, N Prakash, S Blaess, F Edenhofer, C Brodski
J. Neurosci., 2018-01-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Transcription factor-induced lineage programming of noradrenaline and motor neurons from embryonic stem cells.
Authors: Mong J, Panman L, Alekseenko Z, Kee N, Stanton L, Ericson J, Perlmann T
Stem Cells, 2014-03-01;32(3):609-22.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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