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Recombinant Mouse BTNL9 Fc Chimera Protein, CF

Catalog #: 9555-BT Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9555-BT-050
Recombinant Mouse BTNL9 Fc Chimera Protein Bioactivity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse BTNL9 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.75-4.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse BTNL9 protein
Mouse BTNL9
(Asp36-Lys257)
Accession # Q8BJE2-1		IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
Q8BJE2-1
N-terminal Sequence
Analysis
Asp36
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
52 kDa
SDS-PAGE
60-73 kDa, reducing conditions

Product Datasheets

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9555-BT

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9555-BT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse BTNL9 Fc Chimera Protein Bioactivity View Larger

Recombinant Mouse BTNL9 Fc Chimera (Catalog # 9555-BT) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD antibody. The ED50 for this effect is 0.75-4.5 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BTNL9

Butyrophilin-like 9 (BTNL9) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Mouse BTNL9 is a 536 amino acid (aa) type I transmembrane glycoprotein that contains a 35 aa signal sequence, a 222 aa extracellular domain (ECD), a 21 aa transmembrane region and a 258 aa cytoplasmic segment. The ECD features two V-type Ig-like domains and three glycosylation sites (2-4). The ECD of mouse BTNL9 shares ~67% and 91% aa sequence identity with the ECD of human and rat BTNL9, respectively. BTNL9 is expressed in a variety of tissues in humans and mice. Among isolated immune cell types, B cells show the strongest expression (2, 5). Recombinant BTNL9–Fc has been shown to bind to many immune cells including T cells, B cells, macrophages and dendritic cells (6). With this profile of binding, it will not be surprising if BTNL9 joined the panel of butyrophilins with negative regulatory effects on immune cells (5). BTNL9 has been reported to inhibit CD8+ T Cell proliferation (5).

References
  1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523
  2. Arnett, H.A. et al. (2009) Cytokine 46:370.
  3. Chapoval, A.I. et al. (2013) Mol. Immunol. 56:819.
  4. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  5. Arnett, H.A. et al. (2014) Nat Rev Immunol. 14:5596.
  6. Yamazaki, T. et al. (2010) J. Immunol. 185:5907.
Long Name
Butyrophilin-like 9
Entrez Gene IDs
153579 (Human); 237754 (Mouse); 686977 (Rat)
Alternate Names
BTN3; BTNL9; butyrophilin 3; butyrophilin-like 9; butyrophilin-like protein 9; UNQ1900/PRO4346; VDLS1900

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