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Recombinant Mouse CCL12/MCP-5 Protein

Catalog #: 428-P5
5 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
428-P5-025/CF

With Carrier

Catalog # Availability Size / Price Qty
428-P5-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (5)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CCL12/MCP-5 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A. The ED50 for this effect is 5‑30 ng/mL.
Source
E. coli-derived mouse CCL12/MCP-5 protein
Gly23-Gly104
Accession #
Q62401.1
N-terminal Sequence
Analysis
Gly23
Predicted Molecular Mass
9.3 kDa

Product Datasheets

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428-P5 (with carrier)

Product Datasheet
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428-P5/CF (carrier free)

Product Datasheet
COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

428-P5

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

428-P5/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL12/MCP-5

CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid sequence identity in the mature protein). Mouse CCL12 encodes a 104 amino acid residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP-1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR-2 but not CCR-1, CCR-3 or CCR-5.

References
  1. Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
  2. Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.
Entrez Gene IDs
20293 (Mouse)
Alternate Names
C-C motif chemokine 12; CCL12; chemokine (C-C motif) ligand 12; MCP5; MCP-5; Scya12

Citations for Recombinant Mouse CCL12/MCP-5 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Sepsis-trained macrophages promote antitumoral tissue-resident T cells
    Authors: Broquet, A;Gourain, V;Goronflot, T;Le Mabecque, V;Sinha, D;Ashayeripanah, M;Jacqueline, C;Martin, P;Davieau, M;Boutin, L;Poulain, C;Martin, FP;Fourgeux, C;Petrier, M;Cannevet, M;Leclercq, T;Guillonneau, M;Chaumette, T;Laurent, T;Harly, C;Scotet, E;Legentil, L;Ferrières, V;Corgnac, S;Mami-Chouaib, F;Mosnier, JF;Mauduit, N;McWilliam, HEG;Villadangos, JA;Gourraud, PA;Asehnoune, K;Poschmann, J;Roquilly, A;
    Nature immunology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. CCL12 induces trabecular bone loss by stimulating RANKL production in BMSCs during acute lung injury
    Authors: C Ma, J Gao, J Liang, F Wang, L Xu, J Bu, B He, G Liu, R Niu, G Liu
    Experimental & Molecular Medicine, 2023-04-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. -->The CCL2/CCL7/CCL12/CCR2 pathway is substantially and persistently upregulated in mice after traumatic brain injury, and CCL2 modulates the complement system in microglia
    Authors: K Popiolek-B, A Ciechanows, K Ciapa?a, K Pawlik, M Oggioni, D Mercurio, MG De Simoni, J Mika
    Mol Cell Probes, 2020-11-04;54(0):101671.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Chemokines CCL2 and CCL7, but not CCL12, play a significant role in the development of pain-related behavior and opioid-induced analgesia
    Authors: K Kwiatkowsk, K Popiolek-B, A Piotrowska, E Rojewska, K Ciapa?a, W Makuch, J Mika
    Cytokine, 2019-04-16;119(0):202-213.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. The role of CCL21 in recruitment of T-precursor cells to fetal thymi.
    Authors: Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y
    Blood, 2004-09-09;105(1):31-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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