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Recombinant Mouse CCL28 Protein

Catalog #: 533-VI
8 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
533-VI-025/CF

With Carrier

Catalog # Availability Size / Price Qty
533-VI-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (8)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CCL28 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR10. The ED50 for this effect is 0.3‑1 µg/mL.
Source
E. coli-derived mouse CCL28 protein
Ile23-Arg130
Accession #
Q9JIL2
N-terminal Sequence
Analysis
Ile23
Predicted Molecular Mass
12.3 kDa

Product Datasheets

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533-VI (with carrier)

Product Datasheet
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SDS

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533-VI/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

533-VI

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

533-VI/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL28

Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag-1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 108 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 (GPR2 orphan receptor) which is also the receptor for CCL27/CTACK.

References
  1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.
Entrez Gene IDs
56477 (Human); 56838 (Mouse)
Alternate Names
CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC

Citations for Recombinant Mouse CCL28 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. CCL28 Enhances HSV-2 gB-Specific Th1-Polarized Immune Responses against Lethal Vaginal Challenge in Mice
    Authors: Y Yan, K Hu, M Fu, X Deng, X Guan, S Luo, M Zhang, Y Liu, Q Hu
    Vaccines, 2022-08-10;10(8):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Antibody-mediated depletion of CCR10+ EphA3+ cells ameliorates fibrosis in IPF
    Authors: MS Hohmann, DM Habiel, MS Espindola, G Huang, I Jones, R Narayanan, AL Coelho, JM Oldham, I Noth, SF Ma, A Kurkciyan, JL McQualter, G Carraro, B Stripp, P Chen, D Jiang, PW Noble, W Parks, J Woronicz, G Yarranton, LA Murray, CM Hogaboam
    JCI Insight, 2021-06-08;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells
    Authors: P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun
    Aging (Albany NY), 2019-09-26;11(18):7402-7415.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. CCL28-Deficient Mice Have Reduced IgA Antibody-Secreting Cells and an Altered Microbiota in the Colon
    Authors: K Matsuo, D Nagakubo, S Yamamoto, A Shigeta, S Tomida, M Fujita, T Hirata, I Tsunoda, T Nakayama, O Yoshie
    J. Immunol., 2017-12-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin
    Authors: SA Geherin, D Gómez, RA Glabman, G Ruthel, A Hamann, GF Debes
    J. Immunol, 2016-02-05;196(6):2514-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. A "prime-pull" vaccine strategy has a modest effect on local and systemic antibody responses to HIV gp140 in mice.
    Authors: Tregoning, John S, Buffa, Viviana, Oszmiana, Anna, Klein, Katja, Walters, Adam A, Shattock, Robin J
    PLoS ONE, 2013-11-19;8(11):e80559.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Mucosa-associated epithelial chemokine/CCL28 expression in the uterus attracts CCR10+ IgA plasma cells following mucosal vaccination via estrogen control.
    Authors: Cha HR, Ko HJ, Kim ED
    J. Immunol., 2011-08-10;187(6):3044-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells.
    Authors: Hieshima K, Kawasaki Y, Hanamoto H, Nakayama T, Nagakubo D, Kanamaru A, Yoshie O
    J. Immunol., 2004-09-15;173(6):3668-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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