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Recombinant Mouse CCL4/MIP-1 beta Protein

Catalog #: 451-MB
11 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
451-MB-010/CF
451-MB-050/CF

With Carrier

Catalog # Availability Size / Price Qty
451-MB-010
451-MB-050
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Citations (11)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CCL4/MIP-1 beta Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 2.50-25.0 ng/mL.
Source
E. coli-derived mouse CCL4/MIP-1 beta protein
Ala24-Asn92
Accession #
Q5QNV9
N-terminal Sequence
Analysis
Ala24
Predicted Molecular Mass
7.8 kDa

Product Datasheets

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451-MB (with carrier)

Product Datasheet
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SDS

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451-MB/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

451-MB

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

451-MB/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL4/MIP-1 beta

CCL4, also known as macrophage inflammatory protein 1 beta (MIP-1 beta , is a 12 kDa beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells (1, 2). CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium (3 - 8). A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment (6). CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha (9). The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV (10, 11). Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2 (10). In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor (2). Both forms of CCL4 block HIV-1 infection of T cells by inducing the downregulation of CCR5 (10). Mature mouse CCL4 shares 77% and 86% aa sequence identity with human and rat CCL4, respectively.

References
  1. Rot, A. and U.H. von Andrian (2004) Annu. Rev. Immunol. 22:891.
  2. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
  3. Sun, X. et al. (2006) Infec. Immun. 74:5943.
  4. Bisset, L.R. and Schmid-Grendelmeier, P. (2005) Curr. Opin. Pulm. Med. 11:35.
  5. Frangogiannis, N.G. (2004) Inflamm. Res. 53:585.
  6. Bystry, R.S. et al. (2001) Nat. Immunol. 2:1126.
  7. Oliveira, S.H.P. et al. (2002) J. Leukoc. Biol. 71:1019.
  8. Schall, T.J. et al. (1993) J. Exp. Med. 177:1821.
  9. Guan, E. et al. (2001) J. Biol. Chem. 276:12404.
  10. Guan, E. et al. (2002) J. Biol. Chem. 277:32348.
  11. Guan, E. et al. (2004) J. Cell. Biochem. 92:53.
Entrez Gene IDs
6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine); 102117861 (Cynomolgus Monkey)
Alternate Names
ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2

Citations for Recombinant Mouse CCL4/MIP-1 beta Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Neuronal hyperexcitability drives central and peripheral nervous system tumor progression in models of neurofibromatosis-1
    Authors: C Anastasaki, J Mo, JK Chen, J Chatterjee, Y Pan, SM Scheaffer, O Cobb, M Monje, LQ Le, DH Gutmann
    Nature Communications, 2022-05-19;13(1):2785.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Asthma reduces glioma formation by T cell decorin-mediated inhibition of microglia
    Authors: J Chatterjee, S Sanapala, O Cobb, A Bewley, AK Goldstein, E Cordell, X Ge, JR Garbow, MJ Holtzman, DH Gutmann
    Nature Communications, 2021-12-08;12(1):7122.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Basil Polysaccharide Reverses Development of Experimental Model of Sepsis-Induced Secondary Staphylococcus aureus Pneumonia
    Authors: X Chen, Y He, Q Wei, C Wang
    Mediators of Inflammation, 2021-05-18;2021(0):5596339.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Midkine activation of CD8+ T cells establishes a neuron-immune-cancer axis responsible for low-grade glioma growth
    Authors: X Guo, Y Pan, M Xiong, S Sanapala, C Anastasaki, O Cobb, S Dahiya, DH Gutmann
    Nat Commun, 2020-05-01;11(1):2177.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. Astrocytic orosomucoid-2 modulates microglial activation and neuroinflammation
    Authors: M Jo, JH Kim, G Song, M Seo, E Mi Hwang, K Suk
    J. Neurosci, 2017-02-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. The Friend leukaemia virus integration 1 (Fli-1) transcription factor affects lupus nephritis development by regulating inflammatory cell infiltration into the kidney.
    Authors: Sato S, Zhang X
    Clin Exp Immunol, 2014-07-01;177(1):102-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration.
    Authors: Fujihara Y, Takato T, Hoshi K
    Stem Cells, 2014-05-01;32(5):1208-19.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Bv8 regulates myeloid-cell-dependent tumour angiogenesis.
    Authors: Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N
    Nature, 2007-12-06;450(7171):825-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Differential sensitivities of pyrogenic chemokine fevers to CC chemokine receptor 5 antibodies.
    Authors: Tavares E, Minano FJ
    Fundam Clin Pharmacol, 2004-04-01;18(2):163-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Role of the autocrine chemokines MIP-1alpha and MIP-1beta in the metastatic behavior of murine T cell lymphoma.
    Authors: Menten P, Saccani A, Dillen C, Wuyts A, Struyf S, Proost P, Mantovani A, Wang JM, Van Damme J
    J. Leukoc. Biol., 2002-10-01;72(4):780-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats.
    Authors: Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA
    J. Immunol., 2000-03-01;164(5):2650-9.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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