Recombinant Mouse CD36/SR-B3 Fc Chimera Protein, CF

Recombinant Mouse CD36/SR-B3 Fc Chimera Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: CD36/SR-B3

CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N-terminal cytoplasmic domain, a 22 aa N-terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C-terminal transmembrane segment, and a 9 aa C-terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C-terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N-terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 is 94%, 92%, 84% and 84% aa identical to the extracellular loops of rat, hamster, human and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells and hematopoietic cells such as RBC’s, platelets and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).

Related Research Areas

• Alpha Granule Membrane Proteins
• Endothelial Cell Markers
• Immune Regulatory Molecules on Endothelial Cells
• Leukocyte Receptors/Markers in Allergy and Asthma
• Macrophage Activation Markers
• Microglia Activation
• Microglia Activation Markers
• Monocyte Markers
• Mouse Tissue-Resident Macrophage Markers
• Myeloid Lineage Markers
• Platelet Adhesion Molecules
• Red Blood Cells (RBCs)
• Scavenger Receptors
• Toll-Like Receptors
• Uptake and Transport Molecules in VSMC
• Uptake and Transport Molecules on Endothelial Cells
• Uptake of Amyloid beta by Glia Cells
• Uptake of Amyloid beta by Macrophages