Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein
Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein Summary
Product Specifications
| HA (YPYDVPDYA) |
GCN4-IZ | GGGSGGGSGGGS | Mouse CD40 Ligand/TNFSF5 (Met112-Leu260) Accession # P27548 |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8230-CL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
8230-CL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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Recombinant Mouse CD40 Ligand/TNFSF5 (Catalog # 8230-CL) has a molecular weight (MW) of 66.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Reconstitution Calculator
Background: CD40 Ligand/TNFSF5
CD40 Ligand, also known as TNFSF5, CD154, TRAP, or gp39, is a 33-39 kDa type II transmembrane glycoprotein member of the TNF superfamily (1, 2). Mature mouse CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and a 214 aa extracellular region (1). The extracellular domain of mouse CD40 Ligand shares 75% and 93% amino acid (aa) sequence identity with the human and rat proteins, respectively. CD40 Ligand is expressed as a homotrimer on platelets and activated T cells and B cells. It is up-regulated following stimulation of basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds and activates CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial cells, and epithelial cells (3). The 18 kDa soluble form (aa 112-260) arises from proteolytic processing and retains the ability to bind and activate CD40 (4, 5). Monomeric, dimeric, and trimeric forms of soluble CD40 Ligand bind to oligomeric CD40 on cell membranes (2). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (6, 7). CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS (8, 9). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (10, 11).
- Armitage, R.J. et al. (1992) Nature 357:80.
- Hollenbaugh, D. et al. (1992) EMBO J. 11:4313.
- van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
- Graf, D. et al. (1995) Eur. J. Immunol. 25:1749.
- Mazzei, G.J. et al. (1995) J. Biol. Chem. 270:7025.
- Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
- Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
- Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
- Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
- Pamukcu, B. et al. (2011) Ann. Med. 43:331.
- Hassan, G.S. et al. (2012) Immunobiology 217:521.
Citations for Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Targeting BMI-1 in B cells restores effective humoral immune responses and controls chronic viral infection
Authors: A Di Pietro, J Polmear, L Cooper, T Damelang, T Hussain, L Hailes, K O'Donnell, V Udupa, T Mi, S Preston, A Shtewe, U Hershberg, SJ Turner, NL La Gruta, AW Chung, DM Tarlinton, CD Scharer, KL Good-Jacob
Nature Immunology, 2021-11-29;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The Histone Methyltransferase DOT1L Is Essential for Humoral Immune Responses
Authors: L Kealy, A Di Pietro, L Hailes, S Scheer, L Dalit, JR Groom, C Zaph, KL Good-Jacob
Cell Reports, 2020-12-15;33(11):108504.
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Cutting Edge: Quantitative Determination of CD40L Threshold for IL-12 and IL-23 Production from Dendritic Cells
Authors: K Abdi, K Laky, K Padhan, C Petrovas, J Skinner, J Kabat, DW Dorward, J Brzostowsk, EO Long, G Trinchieri, R Varma
J. Immunol., 2018-10-12;0(0):.
Applications: Cell Culture -
Identification and characterisation of the CD40-ligand of Sigmodon hispidus
Authors: MS Russell, A Muralidhar, L Larocque, J Cao, Y Deschambau, J Varga, SN Thulasi Ra, X Li
PLoS ONE, 2018-07-27;13(7):e0199067.
Species: Mouse
Sample Types: Recombinant Protein
Applications: ELISA Developmet -
Mining the Plasma Cell Transcriptome for Novel Cell Surface Proteins
Authors: S Trezise, A Karnowski, PL Fedele, S Mithraprab, Y Liao, K D'Costa, AJ Kueh, MP Hardy, CM Owczarek, MJ Herold, A Spencer, W Shi, SN Willis, SL Nutt, LM Corcoran
Int J Mol Sci, 2018-07-24;19(8):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
B cell activation and plasma cell differentiation are inhibited by de novo DNA methylation
Authors: BG Barwick, CD Scharer, RJ Martinez, MJ Price, AN Wein, RR Haines, APR Bally, JE Kohlmeier, JM Boss
Nat Commun, 2018-05-15;9(1):1900.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Regulation of the DNA Repair Complex during Somatic Hypermutation and Class-Switch Recombination
Authors: A Kumar, A Priya, T Ahmed, C Grundström, N Negi, T Grundström
J. Immunol., 2018-05-04;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Plasma cell differentiation is controlled by multiple cell division-coupled epigenetic programs
Authors: CD Scharer, BG Barwick, M Guo, APR Bally, JM Boss
Nat Commun, 2018-04-27;9(1):1698.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Zinc finger-IRF composite elements bound by Ikaros/IRF4 complexes function as gene repression in plasma cell
Authors: K Ochiai, H Kondo, Y Okamura, H Shima, Y Kurokochi, K Kimura, R Funayama, T Nagashima, K Nakayama, K Yui, K Kinoshita, K Igarashi
Blood Adv, 2018-04-24;2(8):883-894.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
T Follicular Helper Cell-Germinal Center B Cell Interaction Strength Regulates Entry into Plasma Cell or Recycling Germinal Center Cell Fate
Authors: W Ise, K Fujii, K Shiroguchi, A Ito, K Kometani, K Takeda, E Kawakami, K Yamashita, K Suzuki, T Okada, T Kurosaki
Immunity, 2018-04-17;48(4):702-715.e4.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Bach2 Promotes B Cell Receptor-Induced Proliferation of B Lymphocytes and Represses Cyclin-Dependent Kinase Inhibitors
Authors: Y Miura, M Morooka, N Sax, R Roychoudhu, A Itoh-Nakad, A Brydun, R Funayama, K Nakayama, S Satomi, M Matsumoto, K Igarashi, A Muto
J. Immunol., 2018-03-14;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Mesenchymal stem cells-derived exosomes are more immunosuppressive than microparticles in inflammatory arthritis
Authors: S Cosenza, K Toupet, M Maumus, P Luz-Crawfo, O Blanc-Brud, C Jorgensen, D Noël
Theranostics, 2018-02-03;8(5):1399-1410.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The Small Rho GTPase TC10 Modulates B Cell Immune Responses
Authors: M Burbage, SJ Keppler, B Montaner, PK Mattila, FD Batista
J. Immunol., 2017-07-26;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Selenoprotein MsrB1 promotes anti-inflammatory cytokine gene expression in macrophages and controls immune response in vivo
Authors: BC Lee, SG Lee, MK Choo, JH Kim, HM Lee, S Kim, DE Fomenko, HY Kim, JM Park, VN Gladyshev
Sci Rep, 2017-07-11;7(1):5119.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
TLR3-Induced Maturation of Murine Dendritic Cells Regulates CTL Responses by Modulating PD-L1 Trafficking
PLoS ONE, 2016-12-02;11(12):e0167057.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Regulated localization of an AID complex with E2A, PAX5 and IRF4 at the Igh locus
Authors: Thomas Grundström
Mol. Immunol., 2016-11-12;80(0):78-90.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Syk tyrosine kinase is critical for B cell antibody responses and memory B cell survival.
Authors: Ackermann J, Nys J, Schweighoffer E, McCleary S, Smithers N, Tybulewicz V
J Immunol, 2015-04-10;194(10):4650-6.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
IKK-induced NF-kappaB1 p105 proteolysis is critical for B cell antibody responses to T cell-dependent antigen.
Authors: Jacque E, Schweighoffer E, Visekruna A, Papoutsopoulou S, Janzen J, Zillwood R, Tarlinton D, Tybulewicz V, Ley S
J Exp Med, 2014-09-15;211(10):2085-101.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Roles of the kinase TAK1 in CD40-mediated effects on vascular oxidative stress and neointima formation after vascular injury.
Authors: Song Z, Zhu X, Jin R, Wang C, Yan J, Zheng Q, Nanda A, Granger D, Li G
PLoS ONE, 2014-07-22;9(7):e101671.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The TACI receptor regulates T-cell-independent marginal zone B cell responses through innate activation-induced cell death.
Authors: Figgett W, Fairfax K, Vincent F, Le Page M, Katik I, Deliyanti D, Quah P, Verma P, Grumont R, Gerondakis S, Hertzog P, O'Reilly L, Strasser A, Mackay F
Immunity, 2013-09-05;39(3):573-83.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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What is the purpose of the GCN4-IZ part of Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein) (Catalog # 8230-CL)?
CD40 Ligand naturally forms homotrimers, both as membrane bound and soluble forms. GCN4-IZ is a trimerization domain that promotes homotrimerization. We expect #8230-CL to include trimeric species; however, we do not have a structural specification for these products, relying instead on bioassay with a cross-linking antibody such as Anti-Hemagglutinin/HA Peptide antibody (Catalog # MAB060) to confirm consistency in bioactivity.
Reviews for Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein
Average Rating: 4.8 (Based on 4 Reviews)
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Reason for Rating: I used this product in order to culture and expand murine B cells, along with the HA-Tag Antibody
