Home / CD40/TNFRSF5 / Recombinant Mouse CD40/TNFRSF5 Fc Chimera Protein, CF

Recombinant Mouse CD40/TNFRSF5 Fc Chimera Protein, CF

Catalog #: 1215-CD
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1215-CD-050
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Citations (4)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CD40/TNFRSF5 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD40/TNFRSF5 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse CD40 Ligand/TNFSF5 (Catalog # 8230-CL) that produces 50% optimal binding is found to be 100-600 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CD40/TNFRSF5 protein
Mouse CD40
(Val24-Arg193)
Accession # P27512		 DIEGRMD Human IgG1
(Pro100-Lys330) 		6-His tag
N-terminus C-terminus
Accession #
P27512
N-terminal Sequence
Analysis
Val24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
46 kDa (monomer)
SDS-PAGE
58-61 kDa, reducing conditions

Product Datasheets

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1215-CD

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1215-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD40/TNFRSF5

CD40, also known as TNFRSF5, is a 32 kDa type I transmembrane glycoprotein member of the TNF receptor superfamily. Mature mouse CD40 consists of a 174 amino acid (aa) extracellular domain, a transmembrane domain, and a 74 aa cytoplasmic domain (1, 2). The extracellular domain of mouse CD40 shares 58% and 81% aa sequence identity with human and rat CD40, respectively. Multiple mouse CD40 splice variants have been identified including soluble isoforms. CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells (3, 4). Interaction of CD40 with its ligand, CD40 Ligand, leads to the aggregation of CD40 molecules resulting in the initiation of intracellular signaling in both CD40 and CD40 Ligand expressing cells (5). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (6, 7). CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy (8, 9). Dysregulation of CD40/CD40 Ligand expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS (10, 11). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (12, 13).

 

References
  1. Torres, R.M. and E.A. Clark (1992) J. Immunol. 148:620.
  2. Torres, R.M. et al. (1992) J. Immunol. 149:2641.
  3. van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
  4. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
  5. Eissner, G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
  6. Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
  7. Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
  8. Loskog, A.S. and A.G. Eliopoulos (2009) Semin. Immunol. 21:301.
  9. Hangalapura, B.N. et al. (2012) J. Gene Med. 14:416.
  10. Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
  11. Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
  12. Pamukcu, B. et al. (2011) Ann. Med. 43:331.
  13. Hassan, G.S. et al. (2012) Immunobiology 217:521.
Entrez Gene IDs
958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)
Alternate Names
B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5

Citations for Recombinant Mouse CD40/TNFRSF5 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Immuno-PET Imaging of Atherosclerotic Plaques with [89Zr]Zr-Anti-CD40 mAb-Proof of Concept
    Authors: K Poels, M Schreurs, M Jansen, DJ Vugts, TTP Seijkens, GAMS van Dongen, E Lutgens, W Beaino
    Biology, 2022-03-06;11(3):.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: Binding Assay
  2. Dendritic cell-targeted CD40 DNA vaccine suppresses Th17 and ameliorates progression of experimental autoimmune glomerulonephritis
    Authors: Q Li, Q Cao, C Wang, H Nguyen, XM Wang, G Zheng, YM Wang, S Hu, SI Alexander, DCH Harris, Y Wang
    J. Leukoc. Biol., 2019-02-27;0(0):.
    Species: Mouse
    Sample Types: Serum
    Applications: Binding Assay
  3. Functional analysis of recombinant calreticulin fragment 39-272: implications for immunobiological activities of calreticulin in health and disease.
    Authors: Hong C, Qiu X, Li Y
    J. Immunol., 2010-09-20;185(8):4561-9.
    Species: Mouse
    Sample Types:
    Applications: ELISA Developmet
  4. The tumor necrosis factor family receptors RANK and CD40 cooperatively establish the thymic medullary microenvironment and self-tolerance.
    Authors: Akiyama T, Shimo Y, Yanai H, Qin J, Ohshima D, Maruyama Y, Asaumi Y, Kitazawa J, Takayanagi H, Penninger JM, Matsumoto M, Nitta T, Takahama Y, Inoue J
    Immunity, 2008-09-19;29(3):423-37.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay

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