Recombinant Mouse CD47 Fc Chimera Protein, CF Summary
Product Specifications
Also measured by its ability to antagonize mouse red blood cell adhesion to immobilized Recombinant Mouse SIRP alpha /CD172a Fc Chimera (Catalog # 7154-SA). The ED50 for this effect is 2.0-8.0 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
| Mouse CD47 (Gln19-Pro158) Accession # NP_034711 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1866-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: CD47
CD47, also known as Integrin‑Associated Protein (IAP) and OA3, is a 40‑60 kDa variably glycosylated atypical member of the immunoglobulin superfamily (1, 2). Mouse CD47 is an integral membrane protein that consists of a 122 amino acid (aa) extracellular domain (ECD) with a single Ig‑like domain, five membrane-spanning regions with short intervening loops, and a 16 aa C‑terminal cytoplasmic tail (3). Alternate splicing of mouse CD47 generates an additional isoform with an insertion of 21 aa following the Ig‑like domain (3). Within the N‑terminal ECD, mouse CD47 shares 63% and 84% aa sequence identity with human and rat CD47, respectively. A portion of the N‑terminal ECD can by shed from smooth muscle cells by MMP-2‑mediated proteolysis (4). The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells (5, 6). These interactions prevent macrophage‑mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium (5‑8). The CD47-SIRP alpha interaction is species specific, and this lack of cross-species interaction has been implicated in xenotransplantation rejection (16). CD47 associates in cis with Fas on T cells and enhances Fas‑mediated apoptosis; its ligation promotes T cell anergy and dampens Th1 immune responses (9‑11). CD47 also associates in cis with Integrins alpha 4 beta 1, alpha V beta 3, alpha 2b beta 3, and alpha 2 beta 1 which can positively or negatively modulate Integrin-mediated function (2, 12). In the vasculature, CD47 binding by Thrombospondin‑1 inhibits the angiogenic and vasorelaxant effects of nitric oxide (2, 13, 14). On dendritic cells and myeloma cells, CD47 ligation by TSP‑1 induces giant cell formation and osteoclast differentiation (15).
- Sarfati, M. et al. (2009) Curr. Drug Targ. 9:842.
- Isenberg, J.S. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:615.
- Lindberg, F.P. et al. (1993) J. Cell Biol. 123:485.
- Maile, L.A. et al. (2008) Mol. Endocrinol. 22:1226.
- Oldenborg, P.-A. et al. (2000) Science 288:2051.
- Liu, Y. et al. (2002) J. Biol. Chem. 277:10028.
- Stefanidakis, M. et al. (2008) Blood 112:1280.
- de Vries, H.E. et al. (2002) J. Immunol. 168:5832.
- Manna, P.P. et al. (2005) J. Biol. Chem. 280:29637.
- Avice, M.-N. et al. (2001) J. Immunol. 167:2459.
- Bouguermouh, S. et al. (2008) J. Immunol. 180:8073.
- Barazi, H.O. et al. (2002) J. Biol. Chem. 277:42859.
- Isenberg, J.S. et al. (2009) Nitric Oxide 21:52.
- Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.
- Kukreja, A. et al. (2009) Blood 114:3413.
- Wang H. et al. (2007) Blood 109:836.
Citations for Recombinant Mouse CD47 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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CD47 masks pro-phagocytic ligands in cis on tumor cells to suppress antitumor immunity
Authors: Tang, Z;Zhong, MC;Qian, J;Galindo, CC;Davidson, D;Li, J;Zhao, Y;Hui, E;Veillette, A;
Nature immunology
Species: Mouse
Sample Types: Whole Cells
Applications: Binding -
On-target and direct modulation of alloreactive T cells by a nanoparticle carrying MHC alloantigen, regulatory molecules and CD47 in a murine model of alloskin transplantation
Authors: KA Shahzad, X Wan, L Zhang, W Pei, A Zhang, M Younis, W Wang, C Shen
Drug Deliv, 2018-11-01;25(1):703-715.
Species: Mouse
Sample Types: Nanoparticles
Applications: In Vivo -
Direct modulation of myelin-autoreactive CD4+ and CD8+ T cells in EAE mice by a tolerogenic nanoparticle co-carrying myelin peptide-loaded major histocompatibility complexes, CD47 and multiple regulatory molecules
Authors: W Pei, X Wan, KA Shahzad, L Zhang, S Song, X Jin, L Wang, C Zhao, C Shen
Int J Nanomedicine, 2018-06-27;13(0):3731-3750.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Surface modification of nanoparticles enables selective evasion of phagocytic clearance by distinct macrophage phenotypes
Sci Rep, 2016-05-19;6(0):26269.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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