Recombinant Mouse CD48/SLAMF2 Protein, CF

Catalog #: 3327-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
3327-CD-050

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Recombinant Mouse CD48/SLAMF2 Protein, CF Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized rm2B4/Fc Chimera at 5 µg/mL (100 µL/well) can bind rmCD48 with a linear range of 0.15-10 µg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse CD48/SLAMF2 protein
Phe23-Arg216, with a C-terminal 6-His tag

Accession #

BAE96326

N-terminal Sequence
Analysis

Phe23

Predicted Molecular Mass

23.0 kDa

SDS-PAGE

50-60 kDa, reducing conditions

Product Datasheets

3327-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3327-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CD48/SLAMF2

CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily proteins (1 - 3). The mouse CD48 cDNA encodes a 240 amino acid (aa) precursor that includes a 22 aa signal sequence, a 195 aa mature protein that contains one Ig-like V-type domain and one Ig-like C2-type domain, and a 23 aa C-terminal propeptide (4). A soluble form of CD48 has been detected in the serum of lymphoid leukemia and arthritis patients (5). Mouse CD48 shares 51% and 68% aa sequence identity with human and rat CD48, respectively. It shares 18% - 26% aa sequence identity with comparable regions of mouse 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. CD48 is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors (4, 6). Among dendritic cells (DC), CD48 is selectively expressed on circulating myeloid DC and resident bone marrow and thymus DC (7). CD2, 2B4, and heparan sulfate function as CD48 ligands (8 - 10). CD48 is competent to transduce signals and can also trigger signaling through CD2 or 2B4 (8, 11). CD48-CD2 interactions promote T cell activation and class switching to IgG_2a in B cells (8,12). High affinity CD48-2B4 interactions can either promote or inhibit NK cell and cytotoxic T cell (CTL) activation (7, 11, 13, 14). CD48-2B4 ligation does not directly trigger CTL activity but enhances signaling from the T cell receptor (13). CD48-2B4 mediated inhibition of NK cell activity is distinct from MHC I-restricted mechanisms (15). CD48 expressed on NK cells is coactivating, whereas CD48 expressed on other cell types inhibits NK cell activation (14). Both CD48 expressing and nonexpressing cells can be targets of NK cell or CTL-mediated lysis (13, 16).

References

Assarsson, E. et al. (2005) J. Immunol. 175:2045.
Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417.
Loertscher, R. and P. Lavery (2002) Transpl. Immunol. 9:93.
Wong, Y.W. et al. (1990) J. Exp. Med. 171:2115.
Smith, G.M. et al. (1997) J. Clin. Immunol. 17:502.
Keil, M.J. et al. (2005) Cell 121:1109.
Morandi, B. et al. (2005) J. Immunol. 175:3690.
Kato, K. et al. (1992) J. Exp. Med. 176:1241.
Latchman, Y. et al. (1998) J. Immunol. 161:5809.
Ianelli, C.J. et al. (1998) J. Biol. Chem. 273:23367.
Messmer, B. et al. (2006) J. Immunol. 176:4646.
Gao, N. et al. (2005) J. Immunol. 174:4113.
Lee, K.-M. et al. (2003) J. Immunol. 170:4881.
Lee, K.-M. et al. (2006) Blood 107:3181.
McNerney, M.E. et al. (2005) Blood 106:1337.
Lee, K.-M. et al. (2004) J. Exp. Med. 199:1245.

Long Name

SLAM Family Member 2

Entrez Gene IDs

962 (Human); 12506 (Mouse); 245962 (Rat); 102134608 (Cynomolgus Monkey)

Alternate Names

BCM1 surface antigen; BCM1; BCM1Leukocyte antigen MEM-102; BLAST; BLAST1; BLAST1TCT.1; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); CD48 antigen; CD48 molecule; CD48; hCD48; mCD48; MEM-102; SLAMF2